Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria; Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria.
Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.
Physiol Behav. 2023 Jun 1;265:114155. doi: 10.1016/j.physbeh.2023.114155. Epub 2023 Mar 11.
Metabolic and mood-related disturbances can increase the risks of developing adverse mental health problems. The medicinal mushroom, Ganoderma lucidum, is utilized in indigenous medicine to improve quality of life, promote health, and boost vitality. This study investigated the effects of Ganoderma lucidum ethanol extract (EEGL) on feeding behavioral parameters, depressive-like symptoms, and motor activity in Swiss mice. We hypothesized that EEGL would have beneficial effect on metabolic and behavioral outcomes in a dose-related manner. The mushroom was identified and authenticated via techniques of molecular biology. Forty Swiss mice (n = 10/group) of either sex were given distilled water (10 mL/kg) and graded doses of EEGL (100, 200, and 400 mg/kg) orally for 30 days, during which feed and water intake, body weight, neurobehavioral, and safety data were documented. The animals experienced a significant decrease in body weight gain and feed intake while water intake increased in a dose-dependent manner. Furthermore, EEGL significantly diminished immobility time in forced swim test (FST) and tail suspension test (TST). At the 100 and 200 mg/kg, EEGL did not cause significant alteration in motor activity in the open field test (OFT). Meanwhile, an increase in motor activity in male mice without remarkable difference in female mice was observed at the highest dose (400 mg/kg). Eighty percent of mice treated with 400 mg/kg survived till day 30. These findings suggest that EEGL at 100 and 200 mg/kg reduces the amount of weight gained and elicits antidepressant-like effects. Thus, EEGL might be useful for the management of obesity and depressive-like symptoms.
代谢和情绪相关的紊乱会增加出现不良心理健康问题的风险。药用蘑菇灵芝被用于传统医学中，以提高生活质量、促进健康和增强活力。本研究调查了灵芝乙醇提取物（EEGL）对瑞士小鼠摄食行为参数、抑郁样症状和运动活动的影响。我们假设 EEGL 将以剂量相关的方式对代谢和行为结果产生有益影响。该蘑菇通过分子生物学技术进行了鉴定和验证。40 只雌雄各半的瑞士小鼠（n=10/组）分别口服给予蒸馏水（10 mL/kg）和不同剂量的 EEGL（100、200 和 400 mg/kg）30 天，在此期间记录摄食量、饮水量、体重、神经行为和安全性数据。动物的体重增加和摄食量显著减少，而饮水量呈剂量依赖性增加。此外，EEGL 显著减少了强迫游泳试验（FST）和悬尾试验（TST）中的不动时间。在 100 和 200 mg/kg 时，EEGL 未引起旷场试验（OFT）中运动活动的显著改变。然而，在最高剂量（400 mg/kg）时，雄性小鼠的运动活性增加，而雌性小鼠则没有明显差异。80%用 400 mg/kg 治疗的小鼠存活至第 30 天。这些发现表明，EEGL 在 100 和 200 mg/kg 剂量下可减少体重增加，并产生抗抑郁样作用。因此，EEGL 可能有助于肥胖和抑郁样症状的管理。
