Asnaashari Mohammad, Kooshki Niloufar, Salehi Mohammad Mahdi, Azari-Marhabi Saranaz, Amin Moghadassi Hedieh
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
DDS, Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2020 Summer;11(3):243-248. doi: 10.34172/jlms.2020.41. Epub 2020 Jun 21.
is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating from infected root canals. Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% ( =0.011) and when the system was used with sodium hypochlorite, it was 100% ( =0.001). The antibacterial effect of PDT was 90.08% ( =0.011) and it was 99.7% when PDT was combined with sodium hypochlorite ( =0.011). All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.
是一种耐药菌，是感染根管中最常见的菌种。光动力疗法（PDT）是一种利用光敏剂在集中光照下产生的活性氧自由基杀死细菌的方法。此外，作为一种根管消毒的新方法，已经引入了多种冲洗激活系统，其中之一是具有旋转运动和螺旋效应以发挥抗菌作用的GentleFile（GF）。本研究旨在比较上述两种方法在使用和不使用次氯酸钠的情况下，从感染根管中清除细菌的有效性。58颗单根牙被随机分为4个实验组，每组14颗。选取两个样本用于后续的扫描电子显微镜检查，以筛选操作步骤。在每个实验组中，选取10个样本用GF或PDT进行处理；其中3个样本作为阳性对照，另一个样本作为阴性对照。实验组如下：（1）冲洗激活系统，（2）冲洗激活系统+次氯酸钠，（3）PDT，（4）PDT+次氯酸钠。然后对样本进行细菌菌落计数培养。冲洗激活系统处理后的细菌计数下降了99.8%（P = 0.011），当该系统与次氯酸钠一起使用时，下降了100%（P = 0.001）。PDT的抗菌效果为90.08%（P = 0.011），与次氯酸钠联合使用时为99.7%（P = 0.011）。所有这四种方法都可作为根管消毒的补充方法。根据本研究实验组的消毒结果得出结论，激活冲洗系统或PDT等新技术与NaOCl联合使用可改善根管消毒效果，并能在牙髓治疗结果方面提供诸多优势。
