Wang Yanhuang, Xiao Suli, Ma Dianfu, Huang Xiaojing, Cai Zhiyu
School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, China.
Key Laboratory of Stomatology (FMU), Fujian Province University, Fuzhou, Fujian, China.
Photochem Photobiol. 2015 Jul-Aug;91(4):937-41. doi: 10.1111/php.12459. Epub 2015 May 10.
Concentration of sodium hypochlorite (NaOCl) is positively correlated with its effectiveness in root canal disinfection but negatively correlated with its biocompatibility. The objective of this in vitro study was to compare the bactericidal effects among ultrasonic irrigation with different concentration of NaOCl alone or together with photodynamic treatment (PDT) against Enterococcus faecalis (E. faecalis) in infected root canals. One hundred and twenty bovine root canals contaminated with E. faecalis were randomly distributed into 12 groups treated with different disinfection methods: PDT, ultrasonic irrigation with NaOCl at different concentrations (0.5%, 1.0%, 2.0%, 2.5% and 5.25%), and ultrasonic irrigation with NaOCl at different concentrations plus PDT. Data of microorganism load were collected before and after disinfection and analyzed by one-way ANOVA and LSD tests. Significantly enhanced antibacterial effects were noticed in groups treated by PDT plus 2.0% or 2.5% NaOCl irrigation (P < 0.05). No statistical differences existed in bactericidal efficacy among groups of PDT plus ultrasonic irrigation with 2.0%, 2.5% or 5.25% NaOCl, and ultrasonic irrigation with 5.25% NaOCl alone (P > 0.05). Our study confirmed the feasibility to reduce the concentration of NaOCl to a safer level while maintaining its antibacterial efficiency through synergistic effect of PDT with NaOCl ultrasonic irrigation.
次氯酸钠（NaOCl）的浓度与其根管消毒效果呈正相关，但与其生物相容性呈负相关。本体外研究的目的是比较不同浓度的NaOCl单独超声冲洗或联合光动力治疗（PDT）对感染根管内粪肠球菌（E. faecalis）的杀菌效果。120个被粪肠球菌污染的牛根管被随机分为12组，采用不同的消毒方法进行处理：PDT、不同浓度（0.5%、1.0%、2.0%、2.5%和5.25%）的NaOCl超声冲洗，以及不同浓度的NaOCl超声冲洗联合PDT。在消毒前后收集微生物负荷数据，并通过单因素方差分析和LSD检验进行分析。在PDT联合2.0%或2.5% NaOCl冲洗处理的组中，观察到显著增强的抗菌效果（P < 0.05）。PDT联合2.0%、2.5%或5.25% NaOCl超声冲洗组与单独5.25% NaOCl超声冲洗组之间的杀菌效果无统计学差异（P > 0.05）。我们的研究证实了通过PDT与NaOCl超声冲洗的协同作用，在保持抗菌效率的同时将NaOCl浓度降低到更安全水平的可行性。
