Attia Hanaa Rm, Ibrahim Mona Hamed, El-Aziz Shereen H Abd, Hassan Naglaa M, Osman Randa A, Hagag Heba A, Yassa Marianne E, Abdelrahman Amany H, Salama Iman I, Sobeih Mohamed Emam
Clinical & Chemical Pathology Department, Medical Division, National Research Centre, Centre of Excellence, Cairo, Egypt.
Clinical Pathology Department, National Cancer Institute, Cairo University, Cairo, Egypt.
Future Sci OA. 2020 Jun 26;6(7):FSO583. doi: 10.2144/fsoa-2020-0034.
We aimed to investigate gene expression pattern and to explore its methylation heterogeneity in chronic lymphocytic leukemia (CLL).
PATIENTS & METHODS: Eighty one CLL patients and 75 healthy subjects were enrolled and prognostic evaluation of patients was assessed. q-realtime PCR was performed using Applied Biosystems, TaqMan gene expression assay. gene-specific CpG methylation was investigated in real time using pyrosequencing technology.
was differentially expressed in CLL patients. The CpG sites-1, 2 and 3 showed significantly higher mean levels than healthy controls (p = <0.001, 0.007 and 0.009). Significant association between CpG site-1 and CLL has been detected using age-adjusted logistic regression (p < 0.001).
Hypermethylation at gene CpG sites (1,2,3) is a characteristic feature in CLL.
我们旨在研究慢性淋巴细胞白血病（CLL）中的基因表达模式并探索其甲基化异质性。
招募了81例CLL患者和75名健康受试者，并对患者进行了预后评估。使用应用生物系统公司的TaqMan基因表达测定法进行q-实时PCR。使用焦磷酸测序技术实时研究基因特异性CpG甲基化。
在CLL患者中差异表达。CpG位点1、2和3的平均水平显著高于健康对照（p = <0.001、0.007和0.009）。使用年龄调整的逻辑回归检测到CpG位点1与CLL之间存在显著关联（p <0.001）。
基因CpG位点（1、2、3）的高甲基化是CLL的一个特征。
