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基于单细胞RNA测序的NK-AML（M4/M5）中骨髓异质性的特征分析

Characterization of bone marrow heterogeneity in NK-AML (M4/M5) based on single-cell RNA sequencing.

作者信息

Wu Wenqi, Shi Zeyan, Tang Zhongyuan, Li Huiqun, Huang Xiaoke, Liang Xiaolin, Li Jing, Yao Yibin, Zhao Weihua, Wu Meiqing, Luo Jun, Liu Zhenfang

机构信息

Department of Hematology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.

出版信息

Exp Hematol Oncol. 2023 Mar 6;12(1):25. doi: 10.1186/s40164-023-00391-5.

DOI:10.1186/s40164-023-00391-5
PMID:36879313
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9987113/
Abstract

Normal karyotype acute myeloid leukemia (NK-AML) is a heterogeneous hematological malignancy that contains a minor population of self-renewing leukemia stem cells (LSCs), which complicate efforts to achieve long-term survival. We performed single-cell RNA sequencing to profile 39,288 cells from 6 bone marrow (BM) aspirates including 5 NK-AML (M4/M5) patients and 1 healthy donor. The single-cell transcriptome atlas and gene expression characteristics of each cell population in NK-AML (M4/M5) and healthy BM were obtained. In addition, we identified a distinct LSC-like cluster with possible biomarkers in NK-AML (M4/M5) and verified 6 genes using qRT‒PCR and bioinformatic analyses. In conclusion, we utilized single-cell technologies to provide an atlas of NK-AML (M4/M5) cell heterogeneity, composition, and biomarkers with implications for precision medicine and targeted therapies.

摘要

正常核型急性髓系白血病（NK-AML）是一种异质性血液系统恶性肿瘤，其中包含一小部分具有自我更新能力的白血病干细胞（LSC），这使得实现长期生存的努力变得复杂。我们进行了单细胞RNA测序，以分析来自6份骨髓穿刺物的39288个细胞，其中包括5例NK-AML（M4/M5）患者和1名健康供体。获得了NK-AML（M4/M5）和健康骨髓中每个细胞群体的单细胞转录组图谱和基因表达特征。此外，我们在NK-AML（M4/M5）中鉴定出一个具有可能生物标志物的独特LSC样簇，并使用qRT-PCR和生物信息学分析验证了6个基因。总之，我们利用单细胞技术提供了NK-AML（M4/M5）细胞异质性、组成和生物标志物图谱，对精准医学和靶向治疗具有重要意义。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/1057f7864a3f/40164_2023_391_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/84a074428622/40164_2023_391_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/f9d5a66612f3/40164_2023_391_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/67dd76137d60/40164_2023_391_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/13893e9964f3/40164_2023_391_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/1057f7864a3f/40164_2023_391_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/84a074428622/40164_2023_391_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/417d08467134/40164_2023_391_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/c31a3dd02254/40164_2023_391_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/f9d5a66612f3/40164_2023_391_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/67dd76137d60/40164_2023_391_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/13893e9964f3/40164_2023_391_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8878/9987113/1057f7864a3f/40164_2023_391_Fig7_HTML.jpg

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2
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Nat Commun. 2020 Oct 21;11(1):5327. doi: 10.1038/s41467-020-19119-8.
3
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4
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Front Oncol. 2024 Apr 22;14:1365330. doi: 10.3389/fonc.2024.1365330. eCollection 2024.
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