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辐照处理对籽瓜(品种)仁蛋白结构及消化特性的影响

Effects of irradiation treatment on protein structure and digestion characteristics of seed-watermelon ( var.) kernel protein.

作者信息

Li Zhihao, Chu Shang, Wang Ping, Gao Sihai, Li Shugang, Yu Xiongwei

机构信息

Key Laboratory of Fermentation Engineering, Ministry of Education; Glyn O. Phillips Hydrophilic Colloid Research Center, Faculty of Light Industry; School of Food and Biological Engineering, Hubei University of Technology, Wuhan, 430068 Hubei Province People's Republic of China.

College of Life Sciences, Tarim University, Tarim, 843300 Xinjiang People's Republic of China.

出版信息

Food Sci Biotechnol. 2020 Jun 10;29(9):1201-1211. doi: 10.1007/s10068-020-00777-9. eCollection 2020 Sep.

Abstract

This study examined the effects of different doses of irradiation treatments on protein structure and digestion characteristic of seed-watermelon seed kernel protein. The results showed that, the molecular structure of seed-watermelon kernel protein was unfolded after the irradiation treatment, the content of -sheet structure in the secondary structure was decreased, while the content of random coil structure increased. The average particle size of the protein increased, and the hydrophobic group buried in the -sheet structure was exposed hence the surface hydrophobicity increased. Besides, the surface morphology of seed-watermelon protein changed from smooth and flat to coarse and concave, the specific surface area in contact with the aqueous medium increased and its solubility increased, the distribution of peptides in the digesta became wider, and the small molecular weight peptides gradually increased.

摘要

本研究考察了不同剂量辐照处理对籽用西瓜籽仁蛋白结构及消化特性的影响。结果表明,辐照处理后籽用西瓜籽仁蛋白的分子结构展开,二级结构中β-折叠结构含量降低,无规卷曲结构含量增加。蛋白的平均粒径增大,埋于β-折叠结构中的疏水基团暴露,表面疏水性增加。此外,籽用西瓜蛋白的表面形态由光滑平整变为粗糙凹陷,与水介质接触的比表面积增大,溶解度增加,消化物中肽段的分布变宽,小分子肽逐渐增多。

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