Wang Xijian, Gopinath Subash C B, Li Jiexing
Department of Psychiatry, Southeast Corner of the Intersection of Aerospace Avenue and Baomao Expressway, Xi'an Mental Health Center, Chang'an District, Xi'an, 710061 Shaanxi China.
School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Malaysia.
3 Biotech. 2020 Sep;10(9):377. doi: 10.1007/s13205-020-02370-7. Epub 2020 Aug 5.
This work focused on the detection of cortisol on an interdigitated electrode sensor surface using an anti-cortisol antibody. To improve immobilization, antibodies were conjugated with silver nanoparticles and attached to the surface of the sensor. Cortisol interacted in a dose-dependent manner on the antibody-immobilized sensor surface, and current changes were observed. Linear regression analysis was performed by a 3 calculation, and the limit of detection fell into the range of 0.01 and 0.1 ng/mL. The sensitivity of cortisol was calculated to be 0.01 ng/mL and the sensor discriminated against other hormones, namely norepinephrine and progesterone, with higher selectivity for cortisol. This result represented the selective detection of cortisol with high performance, which can help to determine anxiety disorders.
这项工作聚焦于使用抗皮质醇抗体在叉指电极传感器表面检测皮质醇。为了改善固定化效果，抗体与银纳米颗粒偶联并附着在传感器表面。皮质醇在抗体固定的传感器表面以剂量依赖方式相互作用，并观察到电流变化。通过三次计算进行线性回归分析，检测限落在0.01至0.1 ng/mL范围内。计算得出皮质醇的灵敏度为0.01 ng/mL，该传感器对其他激素（即去甲肾上腺素和孕酮）具有区分能力，对皮质醇具有更高的选择性。这一结果代表了对皮质醇的高性能选择性检测，有助于确定焦虑症。
