Depart of Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600 Arau, Perlis, Malaysia; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis, 01000 Kangar, Perlis, Malaysia.
Int J Biol Macromol. 2019 Dec 1;141:564-569. doi: 10.1016/j.ijbiomac.2019.09.012. Epub 2019 Sep 4.
Human papilloma virus (HPV) affects predominantly the genital area, which includes vagina, cervix, penis, vulva scrotum, rectum and anus. Among 100 types of HPV, 14 types are considered to cause the risky cancer. The gene HPV-16 E7 is responsible for the development of cancer with the infected women. Earlier identification of this gene sequence avoids the cancer progression. The targeted HPV-16 E7 sequence was sandwiched by capture and reporter sequences on the carbodiimidazole-modified interdigitated electrode (IDE) surface. Target sequence at 100 f. was paired to the capture sequence immobilized on IDE sensing surface. To this surface, different concentrations of reporter sequence with and without gold rod (GNR) were evaluated. In both cases the detections were attained 1 aM by the reporter sequence pairing and with GNR increments in current were found. This enhancement was found to be 1000 folds, considering the condition was revealed in the absence of reporter. This sandwich detection strategy of capture-target-reporter sequences for HPV-16 detection on the IDE sensing surface helps to diagnose the association of cervical cancer.
人乳头瘤病毒 (HPV) 主要影响生殖器区域，包括阴道、子宫颈、阴茎、外阴、阴囊、直肠和肛门。在 100 种 HPV 类型中，有 14 种被认为会导致高危癌症。HPV-16 E7 基因是导致受感染女性癌症发展的原因。早期识别该基因序列可避免癌症进展。在碳化二亚胺修饰的叉指电极 (IDE) 表面上，通过捕获和报告序列将 HPV-16 E7 靶向序列夹在中间。在 100 fM 时，将 100 fM 的目标序列与固定在 IDE 传感表面上的捕获序列配对。在这种情况下，评估了有无金纳米棒 (GNR) 的不同浓度报告序列。在这两种情况下，通过报告序列配对都可检测到 1 aM 的信号，并且发现随着 GNR 的增加电流也会增加。与没有报告序列的情况相比，这种在 IDE 传感表面上使用捕获-靶标-报告序列的三明治检测策略有助于诊断宫颈癌的相关性。
