M Haghani, V Pouladvand, S M J Mortazavi, M Razavinasab, M Bayat, M Shabani
PhD, Histomorphometry and Stereology Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
MSc, Department of Biochemical, Jiroft University of Medical Sciences, Jiroft, Kerman, Iran.
J Biomed Phys Eng. 2020 Aug 1;10(4):433-440. doi: 10.31661/jbpe.v0i0.560. eCollection 2020 Aug.
Prenatal adverse effects of radiofrequency electromagnetic fields (RF-EMF) exposure on nervous system are an issue of major concern.
Thus, in this study we evaluated the membrane current flow properties of Purkinje neurons after maternal exposure to 900 MHz pulsed RF-EMF.
In this experimental study, during all days of pregnancy, rats in the EMF-exposed group were exposed to 900 MHz pulsed-EMF radiation for 6 h per day. The effects of RF-EMF exposure on the electrophysiological properties of the Purkinje cerebellum neurons from male pups were evaluated by whole-cell patch clamp recordings in current and voltage clamp modes. In voltage-clamp experiments, the holding potential was -60mV, and a depolarizing voltage step (1000 ms duration) was applied from -60 to +50 mV in 10 mV increments at 2s intervals.
The exposure group demonstrated reduced spontaneous firing associated with upward and rightward shift in I/V curve compared to the control rats. Moreover, the peak amplitude of the current for the exposure pups also revealed a significant decrement. The reversal potential was +40 mV and +20 mV for the control and RF-EMF groups, respectively and showed significant differences between the two groups.
The decrease in ion's conductance could be attributed to the observed decrease in the voltage onset of the inward current, peak amplitude and voltage shift.
射频电磁场（RF-EMF）暴露对神经系统的产前不良影响是一个备受关注的问题。
因此，在本研究中，我们评估了母体暴露于900MHz脉冲RF-EMF后浦肯野神经元的膜电流流动特性。
在本实验研究中，在整个孕期，暴露于电磁场组的大鼠每天暴露于900MHz脉冲电磁场辐射6小时。通过全细胞膜片钳记录电流和电压钳模式，评估RF-EMF暴露对雄性幼崽浦肯野小脑神经元电生理特性的影响。在电压钳实验中，钳制电位为-60mV，以2s的间隔，以10mV的增量从-60mV到+50mV施加持续1000ms的去极化电压阶跃。
与对照大鼠相比，暴露组的自发放电减少，I/V曲线向上和向右移动。此外，暴露幼崽的电流峰值幅度也显著下降。对照组和RF-EMF组的反转电位分别为+40mV和+20mV，两组之间存在显著差异。
离子电导率的降低可能归因于观察到内向电流的电压起始、峰值幅度和电压偏移的降低。
