Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, PR China, National Engineering Research Center of Edible Fungi, Shanghai 201403, China.
Biomed Res Int. 2020 Jul 31;2020:2817979. doi: 10.1155/2020/2817979. eCollection 2020.
The study aimed at detecting 13 organic acids (oxalic acid, maleic acid, citric acid, tartaric acid, malic acid, quinic acid, succinic acid, fumaric acid, formic acid, acetic acid, propionic acid, isobutyric acid, and butyric acid) by establishing a high-performance liquid chromatography (HPLC). The analysis was performed using two sugar columns, i.e., SH1011 column and KC-811 column. The optimal conditions were as follows: 4 mmol/L HClO solution as the eluent with UV-visible detector (210 nm), a flow rate of 1 mL/min at the temperature of 60°C, and the injection volume at 10 L. The results showed that all the calibration curves had excellent linearity ( > 0.9991) within the test ranges. The RSD values of the thirteen analytes were lower than 2.94% at three levels, the recoveries were 91.9%-102.0%, the limit of detection (LOD) was between 0.05 and 10.63 g/mL, and the quantification (LOQ) was between 0.10 and 19.53 g/mL. Finally, the proposed methodology was successfully applied for the analysis of organic acids in the culture medium of edible fungi. In conclusion, the study findings proved that the method was sensitive, accurate, reproducible, and could be readily applied to analyze the organic acids in the samples.
本研究旨在通过建立高效液相色谱法（HPLC）检测 13 种有机酸（草酸、马来酸、柠檬酸、酒石酸、苹果酸、奎尼酸、琥珀酸、富马酸、甲酸、乙酸、丙酸、异丁酸和丁酸）。分析采用两种糖柱，即 SH1011 柱和 KC-811 柱。最佳条件为：4mmol/L HClO 溶液作为洗脱液，紫外-可见检测器（210nm），温度为 60°C 时流速为 1mL/min，进样量为 10μL。结果表明，所有校准曲线在测试范围内均具有良好的线性（r ＞0.9991）。13 种分析物在三个水平的 RSD 值均低于 2.94%，回收率为 91.9%-102.0%，检出限（LOD）在 0.05-10.63μg/mL 之间，定量限（LOQ）在 0.10-19.53μg/mL 之间。最后，该方法成功应用于食用真菌培养液中有机酸的分析。综上所述，该方法灵敏、准确、重现性好，可用于样品中有机酸的分析。
