Ogunlaja Aemere, Sharma Vikas, Ghai Meenu, Lin Johnson
Department of Biological Sciences, Redeemer's University, Ede Osun state, Nigeria.
School of Life Sciences, Discipline of Microbiology, University of Kwazulu-Natal, Durban, South Africa.
Mol Biol Res Commun. 2020 Jun;9(2):45-53. doi: 10.22099/mbrc.2019.35242.1449.
Studies on earthworms using molecular markers are rare in Africa except a handful from South Africa. Reports on , an earthworm found in West Africa are available including their metal tolerance and bioaccumulation capacity but their molecular characterization and ecotoxicology studies are scarce. In this study, triplicate specimens were collected from four locations within a petroleum polluted site and one in a control site, ≃1Km away from point of spill. DNA was extracted and 18S rRNA and 16S rRNA genes were amplified and sequenced. DNA methylation of their 18S rRNA gene was determined using Methylation specific PCR (MSP) method. Phylogenetic trees generated for 18S rRNA and 16S rRNA genes grouped within the Eudrilidae family concurrent with its conventional grouping and MSP results indicate no methylation in population from this site.
除了来自南非的少数研究外，非洲利用分子标记对蚯蚓进行的研究很少。关于在西非发现的一种蚯蚓的报告是有的，包括它们的金属耐受性和生物累积能力，但它们的分子特征和生态毒理学研究却很少。在本研究中，从一个石油污染场地内的四个地点和一个距离泄漏点约1公里的对照场地采集了一式三份的样本。提取DNA并对18S rRNA和16S rRNA基因进行扩增和测序。使用甲基化特异性PCR（MSP）方法测定其18S rRNA基因的DNA甲基化。为18S rRNA和16S rRNA基因生成的系统发育树将其归入真蚓科，这与它的传统分类一致，并且MSP结果表明该场地的种群中没有甲基化。
