Sun Wenjun, Liu Li, Yu Ying, Yu Bin, Liang Caice, Ying Hanjie, Liu Dong, Chen Yong
National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210000, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 210000, China.
ACS Omega. 2020 Jul 30;5(31):19737-19746. doi: 10.1021/acsomega.0c02501. eCollection 2020 Aug 11.
In this study, we found that biofilm formation is a critical factor affecting the activity of SJ1 xylanase. Xylanase activity increased 8.8% from 1046.88 to 1147.74 U/mL during SJ1 immobilized fermentation with biofilm formation. Therefore, we carried out the work of genomic analysis and biofilm-related time-series transcriptome analysis of SJ1 for better understanding of the ability of SJ to produce xylanase and biofilm formation. Genome annotation results revealed a complete biofilm polysaccharide component synthesis pathway in SJ1 and five proteins regarding xylanase synthesis. In addition, results of transcriptome analysis revealed that the genes involved in the synthesis of cell wall polysaccharides and amino acid anabolism were highly expressed in the biofilm. Furthermore, the expression levels of major genes in the gluconeogenesis pathway and mitogen-activated protein kinase pathway were examined.
在本研究中，我们发现生物膜形成是影响SJ1木聚糖酶活性的关键因素。在SJ1固定化发酵并形成生物膜的过程中，木聚糖酶活性从1046.88 U/mL增加到1147.74 U/mL，增幅为8.8%。因此，我们开展了SJ1的基因组分析和与生物膜相关的时间序列转录组分析工作，以便更好地了解SJ产生木聚糖酶的能力和生物膜形成情况。基因组注释结果显示，SJ1中存在一条完整的生物膜多糖成分合成途径以及5种与木聚糖酶合成相关的蛋白质。此外，转录组分析结果表明，参与细胞壁多糖合成和氨基酸合成代谢的基因在生物膜中高表达。此外，还检测了糖异生途径和丝裂原活化蛋白激酶途径中主要基因的表达水平。
