Department of Infectious Diseases and Clinical Microbiology, Faculty of Medicine, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdogan University, 53100, Rize, Turkey.
Inflammation. 2021 Feb;44(1):148-159. doi: 10.1007/s10753-020-01316-8.
Acute kidney injury (AKI) resulting from septic shock caused by sepsis is an important health problem encountered at rates of 55-73%. Increasing oxidative stress and inflammation following sepsis is a widely observed condition with rising mortality rates. The purpose of this study was to determine whether perindopril (PER) can prevent sepsis-associated AKI with its antioxidant, anti-inflammatory, and anti-apoptotic effects. The control group received an oral saline solution only for 4 days. Cecal ligation and puncture (CLP)-induced sepsis only was applied to the CLP group, while the CLP + PER (2 mg/kg) received CLP-induced sepsis together with 2 mg/kg PER via the oral route for 4 days before induction of sepsis. Finally, all rats were euthanized by anesthesia and sacrificed. TBARS, total SH levels and NF-κβ, TNF-α, and Caspase-3 expression were then calculated for statistical analysis. TBARS, total SH, NF-kβ/p65, TNF-a, and Caspase-3 levels increased in the CLP group. In contrast, oral administration of PER (2 mg/kg) to septic rats reduced TBARS levels and NF-kβ/p65, TNF-α, and Caspase-3 immunopositivity at biochemical analysis. PER treatment appears to be a promising method for preventing sepsis-induced acute kidney injury through its antioxidant anti-inflammation and anti-apoptotic activities.
脓毒症引起的感染性休克导致的急性肾损伤（AKI）是一种重要的健康问题，发生率为 55-73%。脓毒症后氧化应激和炎症的增加是一种广泛观察到的情况，死亡率不断上升。本研究旨在确定培哚普利（PER）是否具有抗氧化、抗炎和抗凋亡作用，从而预防脓毒症相关 AKI。对照组仅在 4 天内口服生理盐水。CLP 组仅给予盲肠结扎和穿刺（CLP）诱导的脓毒症，而 CLP+PER（2mg/kg）组在诱导脓毒症前 4 天通过口服途径给予 2mg/kg PER，以预防 CLP 诱导的脓毒症。最后，所有大鼠均用麻醉剂安乐死并处死。然后计算 TBARS、总 SH 水平以及 NF-κβ、TNF-α 和 Caspase-3 的表达，进行统计分析。CLP 组的 TBARS、总 SH、NF-kβ/p65、TNF-α 和 Caspase-3 水平升高。相比之下，口服培哚普利（2mg/kg）可降低脓毒症大鼠的 TBARS 水平和 NF-kβ/p65、TNF-α 和 Caspase-3 的免疫阳性率。PER 治疗似乎是一种有前途的方法，可通过其抗氧化、抗炎和抗凋亡作用预防脓毒症引起的急性肾损伤。
