Melbourne Dental School, The University of Melbourne, Carlton, VIC, Australia.
Centre for Oral Health Research, Melbourne Dental School, The University of Melbourne, Carlton, VIC, Australia.
J Periodontal Res. 2021 Jan;56(1):58-68. doi: 10.1111/jre.12793. Epub 2020 Aug 17.
Neutrophils are emerging as a key player in periodontal pathogenesis. The surface expression of cellular markers enables functional phenotyping of neutrophils which have distinct roles in disease states. This study aimed to evaluate the effect of periodontal management on neutrophil phenotypes in peripheral blood in periodontitis patients over one year.
Peripheral blood and the periodontal parameters, mean probing depth and percentage of sites with bleeding on probing (%BOP), were collected from 40 healthy controls and 54 periodontitis patients at baseline and 3-, 6- and 12- months post-treatment. Flow cytometry was used to identify CD11b , CD16b , CD62L and CD66b expression on neutrophils, neutrophil maturation stages as promyelocytes (CD11b CD16b ), metamyelocytes (CD11b CD16b ) and mature neutrophils (CD11b CD16b ), and suppressive neutrophil phenotype as bands (CD16 CD62L ), normal neutrophils (CD16 CD62L ) and suppressive neutrophils (CD16 CD62L ).
CD62L expression decreased with treatment. No differences were observed in neutrophil maturation stages in health or disease upon treatment. Suppressive and normal neutrophils showed a reciprocal relationship, where suppressive neutrophils decreased with treatment and normal neutrophils increased with treatment. In addition, %BOP was associated with suppressive neutrophils.
This study demonstrates that management of periodontitis significantly modifies distinct neutrophil phenotypes in peripheral blood. Suppressive neutrophils may play a role in the pathogenesis of periodontitis. However, their exact role is unclear and requires further investigation.
中性粒细胞在牙周病发病机制中扮演着重要角色。细胞表面标志物的表达使中性粒细胞的功能表型得以鉴定，这些细胞在不同的疾病状态下具有不同的作用。本研究旨在评估牙周治疗对牙周炎患者外周血中性粒细胞表型的影响，随访时间为一年。
本研究共纳入 40 名健康对照者和 54 名牙周炎患者，在基线时以及治疗后 3、6 和 12 个月，收集外周血和牙周参数，包括平均探诊深度和探诊出血百分比（%BOP）。采用流式细胞术鉴定中性粒细胞表面 CD11b、CD16b、CD62L 和 CD66b 的表达，以及中性粒细胞的成熟阶段，包括原始粒细胞（CD11b CD16b ）、早幼粒细胞（CD11b CD16b ）和成熟中性粒细胞（CD11b CD16b ），同时鉴定抑制性中性粒细胞表型（CD16 CD62L ）、正常中性粒细胞（CD16 CD62L ）和抑制性中性粒细胞（CD16 CD62L ）。
随着治疗的进行，CD62L 的表达逐渐降低。在健康或疾病状态下，中性粒细胞的成熟阶段在治疗前后均无差异。抑制性和正常中性粒细胞呈相反关系，即随着治疗的进行，抑制性中性粒细胞减少，而正常中性粒细胞增加。此外，%BOP 与抑制性中性粒细胞有关。
本研究表明，牙周炎的治疗可显著改变外周血中不同的中性粒细胞表型。抑制性中性粒细胞可能在牙周病的发病机制中发挥作用。然而，其确切作用尚不清楚，需要进一步研究。
