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在家蚕幼虫中生产登革热病毒3型样颗粒及其在小鼠中引发体液免疫反应的能力。

Production of dengue virus-like particles serotype-3 in silkworm larvae and their ability to elicit a humoral immune response in mice.

作者信息

Utomo Doddy Irawan Setyo, Pambudi Sabar, Sjatha Fithriyah, Kato Tatsuya, Park Enoch Y

机构信息

Laboratory of Biotechnology, Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan.

Center of Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology (BPPT), Jl. Kawasan Puspiptek, Gedung I LAPTIAB, Kota Tangerang Selatan, Banten, 15314, Indonesia.

出版信息

AMB Express. 2020 Aug 17;10(1):147. doi: 10.1186/s13568-020-01087-3.

DOI:10.1186/s13568-020-01087-3
PMID:32804287
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7431508/
Abstract

To develop monovalent dengue virus-like particle for serotype 3 (DENV-LP/3), we prepared and expressed two structural polyprotein constructs using silkworm and Bm5 cells: DENV-3 Capsid-premembrane-envelope (DENV-3CprME) and premembrane-envelope (DENV-3prME). The expressed PA-tagged 3CprME and 3prME polypeptides were partially purified by PA-tag affinity chromatography and had molecular weights of 85 and 75 kDa, respectively. Expressed proteins were separately verified using the following primary antibodies: the anti-PA tag antibody, DENV premembrane polyclonal antibody, and DENV envelope polyclonal antibody. Transmission electron microscopy revealed that these DENV-3CprME and 3prME formed rough, spherical DENV-LPs (DENV-LP/3CprME and DENV-LP/3prME), respectively, with a diameter of 30-55 nm. The heparin-binding assay demonstrated that these DENV-LPs contained the envelope protein domain III on their surfaces. Both DENV-LPs showed an affinity to sera from human dengue patients and immunized mice. Immunization of mice with DENV-LP/3prME significantly induced the level of antibodies compared with DENV-LP/3CprME. These results indicate that DENV-LP/3prME is suitable as a vaccine candidate compared with DENV-LP/3CprME.

摘要

为了开发3型单价登革病毒样颗粒（DENV-LP/3），我们利用家蚕和Bm5细胞制备并表达了两种结构多蛋白构建体：登革病毒3型衣壳-前膜-包膜（DENV-3CprME）和前膜-包膜（DENV-3prME）。通过PA标签亲和层析对表达的带有PA标签的3CprME和3prME多肽进行了部分纯化，其分子量分别为85 kDa和75 kDa。使用以下一抗分别对表达的蛋白进行验证：抗PA标签抗体、登革病毒前膜多克隆抗体和登革病毒包膜多克隆抗体。透射电子显微镜显示，这些DENV-3CprME和3prME分别形成了粗糙的球形登革病毒样颗粒（DENV-LP/3CprME和DENV-LP/3prME），直径为30 - 55 nm。肝素结合试验表明，这些登革病毒样颗粒在其表面含有包膜蛋白结构域III。两种登革病毒样颗粒均与人登革热患者血清和免疫小鼠血清具有亲和力。与DENV-LP/3CprME相比，用DENV-LP/3prME免疫小鼠显著诱导了抗体水平。这些结果表明，与DENV-LP/3CprME相比，DENV-LP/3prME适合作为候选疫苗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/46e2d1e5a490/13568_2020_1087_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/9ff0b5a53fc6/13568_2020_1087_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/589d2971907f/13568_2020_1087_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/900ea500975f/13568_2020_1087_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/3da6191225d1/13568_2020_1087_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/6bfda5a1c6f1/13568_2020_1087_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/46e2d1e5a490/13568_2020_1087_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/9ff0b5a53fc6/13568_2020_1087_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/589d2971907f/13568_2020_1087_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/900ea500975f/13568_2020_1087_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/3da6191225d1/13568_2020_1087_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/6bfda5a1c6f1/13568_2020_1087_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4c3e/7431508/46e2d1e5a490/13568_2020_1087_Fig6_HTML.jpg

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Sci Rep. 2020 Mar 9;10(1):4297. doi: 10.1038/s41598-020-60309-7.
2
Re-emergence of dengue virus serotype 3 infections in Gabon in 2016-2017, and evidence for the risk of repeated dengue virus infections.2016-2017 年加蓬再次出现登革热病毒 3 型感染，以及重复感染登革热病毒风险的证据。
Int J Infect Dis. 2020 Feb;91:129-136. doi: 10.1016/j.ijid.2019.12.002. Epub 2019 Dec 7.
3
Dengue proteins with their role in pathogenesis, and strategies for developing an effective anti-dengue treatment: A review.
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Front Microbiol. 2023 Jun 7;14:1065609. doi: 10.3389/fmicb.2023.1065609. eCollection 2023.
4
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5
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6
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J Virol. 2018 Aug 29;92(18). doi: 10.1128/JVI.01023-18. Print 2018 Sep 15.
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