Nasrin Fahmida, Tsuruga Kenta, Utomo Doddy Irawan Setyo, Chowdhury Ankan Dutta, Park Enoch Y
Laboratory of Biotechnology, Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Laboratory of Biotechnology, Department of Agriculture, Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.
Biosensors (Basel). 2021 Oct 7;11(10):376. doi: 10.3390/bios11100376.
The treatment for mosquito-borne viral diseases such as dengue virus (DENV), zika virus (ZIKV), and chikungunya virus (CHIKV) has become difficult due to delayed diagnosis processes. In addition, sharing the same transmission media and similar symptoms at the early stage of infection of these diseases has become more critical for early diagnosis. To overcome this, a common platform that can identify the virus with high sensitivity and selectivity, even for the different serotypes, is in high demand. In this study, we have attempted an electrochemical impedimetric method to detect the ZIKV, DENV, and CHIKV using their corresponding antibody-conjugated sensor electrodes. The significance of this method is emphasized on the fabrication of a common matrix of gold-polyaniline and sulfur, nitrogen-doped graphene quantum dot nanocomposites (Au-PAni-N,S-GQDs), which have a strong impedimetric response based only on the conjugated antibody, resulting in minimum cross-reactivity for the detection of various mosquito-borne viruses, separately. As a result, four serotypes of DENV and ZIKV, and CHIKV have been detected successfully with an LOD of femtogram mL.
由于诊断过程的延迟，登革热病毒（DENV）、寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）等蚊媒病毒疾病的治疗变得困难。此外，这些疾病在感染初期共享相同的传播媒介且症状相似，这使得早期诊断变得更加关键。为了克服这一问题，人们迫切需要一个能够以高灵敏度和选择性识别病毒的通用平台，即使对于不同血清型也是如此。在本研究中，我们尝试了一种电化学阻抗法，使用相应的抗体偶联传感电极来检测ZIKV、DENV和CHIKV。该方法的重要性在于制备了一种金-聚苯胺和硫、氮掺杂石墨烯量子点纳米复合材料（Au-PAni-N,S-GQDs）的通用基质，该基质仅基于偶联抗体就具有强烈的阻抗响应，从而在分别检测各种蚊媒病毒时具有最小的交叉反应性。结果，成功检测到了DENV的四种血清型、ZIKV和CHIKV，检测限为飞克/毫升。
