Sproll M W, Spindler E, Anderer F A
Friedrich-Miescher-Laboratorium, Max-Planck-Gesellschaft, Tübingen, F.R.G.
Immunol Lett. 1988 Jan;17(1):29-33. doi: 10.1016/0165-2478(88)90097-1.
In vitro immunization procedures, using sera of athymic mice bearing human WOC ovarian tumors or CM III mammary tumors as immunizing antigen, induced a highly efficient formation of mABs (44% of antibody-producing clones) reacting with human ovarian and/or mammary tumor cells. More than half of these mABs showed cross-reactivity with mouse cell lines. Immunogenicity of normal mouse components in the sera from tumor bearers can be excluded since control immunization with sera of normal athymic mice yielded no mABs reacting with mouse or human cell lines. Furthermore, immunization with sera from tumor bearers did not induce mABs only reacting with mouse cells since 20% of the antibody-producing clones showed an exclusive specificity for the human tumor cells. On the basis of these results we concluded that the human-mouse cross-reacting mABs were induced by circulating human TAA with epitopes shared by mouse cellular components.
采用携带人WOC卵巢肿瘤或CM III乳腺肿瘤的无胸腺小鼠血清作为免疫抗原的体外免疫程序，诱导产生了高效的单克隆抗体（44%的抗体产生克隆），这些抗体可与人卵巢和/或乳腺肿瘤细胞发生反应。其中一半以上的单克隆抗体与小鼠细胞系具有交叉反应性。肿瘤携带者血清中正常小鼠成分的免疫原性可以排除，因为用正常无胸腺小鼠血清进行对照免疫未产生与小鼠或人细胞系发生反应的单克隆抗体。此外，用肿瘤携带者血清进行免疫并未诱导出仅与小鼠细胞发生反应的单克隆抗体，因为20%的抗体产生克隆对人肿瘤细胞具有专一特异性。基于这些结果，我们得出结论，人鼠交叉反应性单克隆抗体是由循环中的人肿瘤相关抗原诱导产生的，这些抗原具有与小鼠细胞成分共有的表位。
