Department of Traditional Chinese Medicine, Zhejiang Pharmaceutical College, No.888 Yinxian Road, 315100, Ningbo, Zhejiang, People's Republic of China.
Department of Traditional Chinese Medicine, Zhejiang Pharmaceutical College, No.888 Yinxian Road, 315100, Ningbo, Zhejiang, People's Republic of China.
J Ethnopharmacol. 2021 Jan 10;264:113245. doi: 10.1016/j.jep.2020.113245. Epub 2020 Aug 14.
Fritillariae Thunbergii Flos (FTF) included in the Chinese Pharmacopoeia (1977 Edition) is a Chinese medicinal herb traditionally used to treat bronchitis. In recent years, it has been applied in the treatment of lung cancer. However, the molecular mechanism remains largely unknown.
The screening of bioactive compounds, acquisition of drug targets, network construction, and experimental validation in vivo were combined to explored the mechanism of FTF in the treatment of lung carcinoma with regards to systems pharmacology.
The network Lung Cancer Pathway consisted of 114 nodes (44 compounds and 70 potential targets) and 361 edges, as well as modules that included inflammatory response, angiogenesis, negative regulation of the apoptotic process, and positive regulation of cell proliferation and migration. It was examined by conducting experiments that involved the administration of ethanol-based extracts of FTF in Lewis lung carcinoma mice. The extracts exerted excellent anti-lung cancer effects in vivo by significantly inhibiting tumor proliferation, thereby extending the survival period of tumor-bearing mice. Moreover, FTF induced the downregulation of PIK3CG, Bcl-2, eNOS, VEGF, p-STAT3, and STAT3 genes in tumor-bearing mice.
The findings of the present study verify the therapeutic effects and mechanism of FTF on lung cancer and provide a theoretical basis to support the comprehensive utilization of FTF resources.
《中国药典》（1977 年版）收载的贝母属植物浙贝母是一种传统的中药，用于治疗支气管炎。近年来，它已被应用于肺癌的治疗。然而，其分子机制在很大程度上仍不清楚。
采用系统药理学方法，结合生物活性化合物的筛选、药物靶点的获取、网络构建和体内实验验证，探讨浙贝母治疗肺癌的作用机制。
构建了包含 114 个节点（44 个化合物和 70 个潜在靶点）和 361 条边的网络肺癌通路，以及包括炎症反应、血管生成、凋亡过程的负调控、细胞增殖和迁移的正调控等模块。通过给Lewis 肺癌小鼠灌胃浙贝母醇提物实验进行了验证，醇提物在体内表现出良好的抗癌作用，显著抑制肿瘤增殖，从而延长荷瘤小鼠的生存期。此外，浙贝母还能诱导荷瘤小鼠中 PIK3CG、Bcl-2、eNOS、VEGF、p-STAT3 和 STAT3 基因的下调。
本研究结果验证了浙贝母治疗肺癌的疗效和机制，为支持浙贝母资源的综合利用提供了理论依据。
