Zheng Wenwen, Zhao Zhongxin, Tian Li, Liu Lele, Xu Tong, Wang Xianwei, He Hongbin, Xia Xianzhu, Zheng Ye, Wei Yurong, Zheng Xuexing
Department of Virology, School of Public Health, Cheeloo College of Medicine, Shandong University, Jinan, China.
College of Life Sciences, Shandong University, Qingdao, China.
Transbound Emerg Dis. 2021 May;68(3):1353-1362. doi: 10.1111/tbed.13796. Epub 2020 Sep 5.
Bovine ephemeral fever (BEF), caused by the bovine ephemeral fever virus (BEFV), is associated with an acute febrile infection in cattle and widespread in tropical and subtropical areas, leading to great economic losses to cattle and milk industry. However, no efficacious BEF vaccine is currently available in China. Herein, we generated a recombinant rabies virus (RABV) expressing BEFV glycoprotein (LBNSE-BG), utilizing a reverse genetics system based on the recombinant rabies virus strain LBNSE. It was found that mice immunized with LBNSE-BG produced robust neutralizing antibodies against both BEFV and RABV, and developed complete protection from lethal RABV challenge. Further studies showed that LBNSE-BG activated more dendritic cells (DCs), B cells and T cells in immunized mice than the parent virus LBNSE. Collectively, these findings demonstrate that the recombinant LBNSE-BG described here has the potential to be developed as a cost-effective and efficacious bivalent vaccine for cattle use in endemic areas of BEF and rabies.
牛流行热（BEF）由牛流行热病毒（BEFV）引起，与牛的急性发热感染有关，在热带和亚热带地区广泛流行，给养牛业和奶制品行业造成巨大经济损失。然而，目前中国尚无有效的牛流行热疫苗。在此，我们利用基于重组狂犬病病毒株LBNSE的反向遗传系统，构建了一种表达BEFV糖蛋白的重组狂犬病病毒（RABV）（LBNSE-BG）。结果发现，用LBNSE-BG免疫的小鼠产生了针对BEFV和RABV的强大中和抗体，并对致死性RABV攻击产生了完全保护。进一步研究表明，与亲本病毒LBNSE相比，LBNSE-BG在免疫小鼠中激活了更多的树突状细胞（DC）、B细胞和T细胞。总体而言，这些发现表明，本文所述的重组LBNSE-BG有潜力被开发成为一种经济有效且高效的二价疫苗，用于牛流行热和狂犬病流行地区的牛。
