Zhuang Hongjun, Zhao Mengyao, Ding Shenglong, Liu Lingyan, Yuan Wei, Jiang Liping, Han Xuemin, Jiang Libo, Yi Tao
Department of Chemistry, Fudan University, Shanghai 200438, P. R. China.
Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, P. R. China.
ACS Appl Mater Interfaces. 2020 Sep 2;12(35):38906-38917. doi: 10.1021/acsami.0c08389. Epub 2020 Aug 20.
Manganese dioxide (MnO) nanostructures have aroused great interest among analytical and biological medicine researchers as a unique type of tumor microenvironment (TME)-responsive nanomaterial. However, reliable approaches for synthesizing yolk-shell nanostructures (YSNs) with mesoporous MnO shell still remain exciting challenges. Herein, a YSN (size, ∼75 nm) containing a mesoporous MnO shell and Er-doped upconversion/downconversion nanoparticle (UCNP) core with a large cavity is demonstrated for the first time. This nanostructure not only integrates diverse functional components including MnO, UCNPs, and YSNs into one system but also endows a size-controllable hollow cavity and thickness-tunable MnO layers, which can load various guest molecules like photosensitizers, methylene blue (MB), and the anticancer drugs doxorubicin (DOX). NIR-II fluorescence and photoacoustic (PA) imaging from UCNP and MB, respectively, can monitor the enrichment of the nanomaterials in the tumors for guiding chemo-photodynamic therapy (PDT) . In the TME, degradation of the mMnO shell by HO and GSH not only generates Mn for tumor-specific T-MR imaging but also releases O and drugs for tumor-specific treatment. The result confirmed that imaging-guided enhanced chemo-PDT combination therapy that benefited from the unique structural features of YSNs could substantially improve the therapeutic effectiveness toward malignant tumors compared to monotherapy.
二氧化锰（MnO）纳米结构作为一种独特的肿瘤微环境（TME）响应型纳米材料，引起了分析和生物医药研究人员的极大兴趣。然而，合成具有介孔MnO壳的蛋黄壳纳米结构（YSNs）的可靠方法仍然是具有挑战性的课题。在此，首次展示了一种尺寸约为75 nm的YSN，其包含介孔MnO壳和具有大空腔的掺铒上转换/下转换纳米颗粒（UCNP）核。这种纳米结构不仅将包括MnO、UCNPs和YSNs在内的多种功能成分整合到一个系统中，还赋予了尺寸可控的中空腔和厚度可调的MnO层，可负载各种客体分子，如光敏剂、亚甲蓝（MB）和抗癌药物阿霉素（DOX）。分别来自UCNP和MB的近红外二区（NIR-II）荧光和光声（PA）成像可以监测纳米材料在肿瘤中的富集情况，以指导化学-光动力疗法（PDT）。在TME中，介孔MnO（mMnO）壳被过氧化氢（HO）和谷胱甘肽（GSH）降解，不仅产生用于肿瘤特异性磁共振成像（T-MR）的锰，还释放氧气和药物用于肿瘤特异性治疗。结果证实，与单一疗法相比，受益于YSNs独特结构特征的成像引导增强化学-光动力联合疗法可显著提高对恶性肿瘤的治疗效果。
