Chiang Chung-Jen, Hu Mu-Chen, Chao Yun-Peng
Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung 40724, Taiwan.
J Agric Food Chem. 2020 Aug 19;68(33):8883-8889. doi: 10.1021/acs.jafc.0c03671. Epub 2020 Aug 4.
Enzymes have a wide range of applications in many sectors of the industry, and the market value has skyrocketed in recent years. Glucose and glycerol are two renewable carbon sources of importance. Therefore, it is appealing to produce recombinant enzymes with these carbon substrates on the basis of economic viability. In this study, glycerol metabolism and glucose metabolism in (. ) were manipulated in a systematic way. In addition, glutamate (Glu) was used for replacement of yeast extract to reduce the cost and the quality-variation problem. A strategy was further developed to incorporate Glu into the central metabolism. The engineered strain finally enabled efficient co-utilization of glucose and glycerol and improved biomass and protein production by 4.3 and 8.2-folds, respectively. The result illustrates that this proposed approach is promising for effective production of recombinant proteins.
酶在许多工业领域有着广泛的应用，近年来其市场价值急剧飙升。葡萄糖和甘油是两种重要的可再生碳源。因此，基于经济可行性，利用这些碳底物生产重组酶具有吸引力。在本研究中，对（.）中的甘油代谢和葡萄糖代谢进行了系统调控。此外，使用谷氨酸（Glu）替代酵母提取物以降低成本和质量变异问题。进一步开发了一种将Glu纳入中心代谢的策略。工程菌株最终实现了对葡萄糖和甘油的高效共利用，生物量和蛋白质产量分别提高了4.3倍和8.2倍。结果表明，该方法在有效生产重组蛋白方面具有前景。
