Mendes Bruna Novaes, Bridi Enrico Coser, França Fabiana Mantovani Gomes, Turssi Cecilia Pedroso, do Amaral Flávia Lucisano Botelho, Basting Rosanna Tarkany, de Oliveira Sousa Ilza Maria, da Silva Tânia Mara, Gonçalves Sérgio Eduardo de Paiva, Foglio Mary Ann, Basting Roberta Tarkany
São Leopoldo Mandic Research Institute, Rua José Rocha Junqueira 13, Bairro Swift, Campinas CEP: 13045-755, São Paulo, Brazil.
Faculty of Pharmaceutical Sciences, University of Campinas, P.O. Box 6029, 13083-859 Campinas, São Paulo, Brazil.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111235. doi: 10.1016/j.msec.2020.111235. Epub 2020 Jun 23.
The aim of this paper was to evaluate the physical properties and the long-term bond strength of a 2.5% polyphenol-enriched extract of Arrabidaea chica (AC) incorporated into both the phosphoric acid and the primer of a three-step total-etch adhesive, or into an aqueous solution as a dentin pretreatment. Fifty dentin surfaces received the treatments (n = 10): CON (control) - application of the three-step adhesive system (Adper Scotchbond Multipurpose, 3M ESPE); WAT - distilled water used as a pretreatment after dentin etching and before application of the adhesive system; ACPA - AC incorporated into the phosphoric acid; ACW - dentin pre-treatment with AC incorporated into an aqueous solution after etching; ACP - AC incorporated into the primer. Microtensile bond strength tests were performed after 24 h, 6 and 12 months of storage. Slices from the resin-dentin interface were obtained for scanning electron microscopy analysis of the hybrid layer. Degree of conversion of AC incorporated into the primer was evaluated. The particle size, polydispersity index and zeta potential of all the solutions prepared by incorporating AC (phosphoric acid, primer and distilled water) were measured by dynamic light scattering, which brought about changes after incorporation. Degree of conversion of the primer was not affected after incorporating AC. ACP showed lower microtensile bond strength values than the other groups. Bond strength decreased after 6 months of storage, stabilizing at the 12-month evaluation. Therefore, use of AC incorporated into the primer led to lower bond strength values, since AC modified the physical properties (particle size, polydispersity index and zeta potential) of the primer, but did not change the degree of conversion. Application of AC as a dentin pretreatment did not affect bond strength or the micromorphological characteristics of the hybrid layer.
本文旨在评估掺入三步全酸蚀粘接剂的磷酸和底胶中，或作为牙本质预处理剂掺入水溶液中的2.5%富含多酚的紫葳科植物提取物（AC）的物理性能和长期粘接强度。五十个牙本质表面接受了以下处理（n = 10）：CON（对照组）——应用三步粘接系统（Adper Scotchbond Multipurpose，3M ESPE）；WAT——牙本质酸蚀后、粘接系统应用前用蒸馏水进行预处理；ACPA——AC掺入磷酸中；ACW——酸蚀后用掺入AC的水溶液进行牙本质预处理；ACP——AC掺入底胶中。在储存24小时、6个月和12个月后进行微拉伸粘接强度测试。获取树脂-牙本质界面的切片用于混合层的扫描电子显微镜分析。评估掺入底胶中的AC的转化率。通过动态光散射测量掺入AC（磷酸、底胶和蒸馏水）制备的所有溶液的粒径、多分散指数和zeta电位，掺入后这些参数发生了变化。掺入AC后底胶的转化率未受影响。ACP组的微拉伸粘接强度值低于其他组。储存6个月后粘接强度下降，在12个月评估时趋于稳定。因此，将AC掺入底胶会导致较低的粘接强度值，因为AC改变了底胶的物理性能（粒径、多分散指数和zeta电位），但未改变转化率。将AC作为牙本质预处理剂应用不会影响粘接强度或混合层的微观形态特征。
