Du Jiawei, Zhang Ying, Jin Zhangya, Wu Hao'an, Cang Jiehui, Shen Yuqing, Miao Fengqin, Zhang Aifeng, Zhang Yu, Zhang Jianqiong, Teng Gaojun
Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Medical School, Southeast University, Nanjing, China.
State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China.
Mater Sci Eng C Mater Biol Appl. 2020 Nov;116:111188. doi: 10.1016/j.msec.2020.111188. Epub 2020 Jun 11.
Tumor metastasis to brain is the main clinical manifestation of patients with advanced breast cancer, leading to poor survival prognosis. In order to detect the early incidence of brain metastasis, it is urgent to develop hypersensitive contrast agents for multimode imaging. In this study, PEG-phospholipids coated, a phage play derived peptide, BRBP1 peptide modified ultra-small iron oxide nanoparticles were prepared for targeted NIRF and MR imaging of breast cancer brain metastasis. The nanoparticles showed 10 nm core-shell, high relaxivity values and photon emission efficiency in vitro. The nanoparticles offered a T2 contrast imaging effect and near-infrared fluorescent signal enhancement. Compared with control peptide modified nanoparticles, the MR/NIRF imaging signal of BRBP1-modified nanoparticles in tumor tissue was significantly enhanced, which should be induced by the targeting ability of BRBP1 peptide. These results indicated that BRBP1-SPIO@mPEG (DiR) nanoparticles could be applied as an effective targeted delivery system for diagnosis of breast cancer brain metastasis.
肿瘤脑转移是晚期乳腺癌患者的主要临床表现，导致生存预后较差。为了检测脑转移的早期发生，迫切需要开发用于多模态成像的超灵敏造影剂。在本研究中，制备了聚乙二醇磷脂包被、噬菌体展示衍生肽BRBP1肽修饰的超小氧化铁纳米颗粒，用于乳腺癌脑转移的靶向近红外荧光（NIRF）和磁共振成像（MR）。纳米颗粒在体外显示出10纳米的核壳结构、高弛豫率值和光子发射效率。纳米颗粒提供了T2对比成像效果和近红外荧光信号增强。与对照肽修饰的纳米颗粒相比，BRBP1修饰的纳米颗粒在肿瘤组织中的MR/NIRF成像信号显著增强，这应该是由BRBP1肽的靶向能力诱导的。这些结果表明，BRBP1-SPIO@mPEG（DiR）纳米颗粒可作为诊断乳腺癌脑转移的有效靶向递送系统。
