Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China; Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University, 510642, Guangdong, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou, 510642, China; Ministry of Agriculture Key Laboratory of Tropical Agricultural Environment, South China Agricultural University, Guangzhou, 510642, China; Guangdong Engineering Research Center for Disposal and Resource Utilization of Animal Wastes, Yunfu, Xinxing, 527400, China.
Environ Pollut. 2020 Oct;265(Pt A):114202. doi: 10.1016/j.envpol.2020.114202. Epub 2020 Feb 26.
Poultry manure is a reservoir for antibiotics and antibiotic resistance genes and composting is an effective biological treatment for manure. This study explored the effect of using two methods of adding a complex microbial agent to the composting of laying-hen manure on doxycycline degradation and tetracycline resistance genes elimination. The results showed that incorporating a complex microbial agent at 0.8% (w/w) on the 0 and 11th day (group MT2) effectively degraded doxycycline with a final degradation rate of 46.83 ± 0.55%. The half-life of doxycycline in this group was 21.90 ± 0.00 days and was significantly lower than that of group MT1 (1.6% (w/w) complex microbial agent added on the 0 day) and group DT (compost without complex microbial agent). But there was no significant difference in the final degradation rate of doxycycline between group DT and group MT1. The addictive with the complex microbial agent changed the microbial community structure. Bacteroidetes, Firmicutes and Proteobacteria were the dominant phyla during composting. Aerococcus, Desemzia, Facklamia, Lactobacillus, Streptococcus, and Trichococcus were the bacteria related to the degradation of doxycycline. Moreover, the incorporation of a complex microbial agent could decrease the risk on spreading tetracycline resistance genes. The single addition promoted the elimination of tetM, whose possible hosts were Enterococcus, Lactobacillus, Staphylococcus, and Trichococcus. Adding the complex microbial agent twice promoted the elimination of tetX, which was related to the low abundance of Chryseobacterium, Flavobacterium and Neptunomonas in group MT2. Redundancy analysis showed that the bacterial community, residual doxycycline and physiochemical properties have a potential effect on the variation in tetracycline resistance genes levels. Overall, adding the complex microbial agent twice is an effective measure to degrade doxycycline.
家禽粪便是抗生素和抗生素抗性基因的储存库，堆肥是一种有效的粪便生物处理方法。本研究探索了在蛋鸡粪堆肥中使用两种添加复合微生物制剂方法对强力霉素降解和四环素抗性基因消除的影响。结果表明，在第 0 天和第 11 天（MT2 组）以 0.8%（w/w）的比例添加复合微生物制剂可有效降解强力霉素，最终降解率为 46.83±0.55%。该组强力霉素的半衰期为 21.90±0.00 天，明显低于 MT1 组（第 0 天添加 1.6%（w/w）复合微生物制剂）和 DT 组（未添加复合微生物制剂的堆肥）。但 DT 组和 MT1 组强力霉素的最终降解率无显著差异。添加复合微生物制剂改变了微生物群落结构。在堆肥过程中，拟杆菌门、厚壁菌门和变形菌门是主要的门。粪肠球菌、德斯美西亚菌、法克氏菌、乳杆菌、链球菌和毛螺菌属是与强力霉素降解相关的细菌。此外，添加复合微生物制剂可以降低四环素抗性基因传播的风险。单一添加促进了 tetM 的消除，其可能的宿主是肠球菌、乳杆菌、葡萄球菌和毛螺菌属。两次添加复合微生物制剂促进了 tetX 的消除，这与 Chryseobacterium、Flavobacterium 和 Neptunomonas 在 MT2 组中的低丰度有关。冗余分析表明，细菌群落、残留强力霉素和理化性质对四环素抗性基因水平的变化有潜在影响。总体而言，两次添加复合微生物制剂是一种有效降解强力霉素的措施。
