Makler M T, Bakke A C, Piper R C
Laboratory and Research Service, Veterans Administration Medical Center, Portland, OR 97201.
J Immunol Methods. 1988 Apr 6;108(1-2):137-43. doi: 10.1016/0022-1759(88)90412-7.
This report describes the use of a fluorescent activated cell sorter (FACS) in combination with an enzyme immunosorbent assay (EIA). This combination of techniques expands the versatility of the flow cytometer. It introduces a new set of fluorescent enzyme products for antigen detection. These highly substantive fluorescent compounds permit the flow cytometer to quantify cell-EIA reactions and also to delineate subpopulations of cells with different quantities of surface antigens. Because the FEIA product is colored, as well as fluorescent, a simple light microscope may also be used to define the distribution of the label on the cell surface. These techniques have been applied to the examination of antigens on human erythrocytes, human T cell lymphoma cells (H9), and to surface markers on the tissue culture cell line K562.
本报告描述了荧光激活细胞分选仪（FACS）与酶免疫吸附测定（EIA）相结合的应用。这种技术组合扩展了流式细胞仪的多功能性。它引入了一组用于抗原检测的新型荧光酶产品。这些高度实质性的荧光化合物使流式细胞仪能够量化细胞-EIA反应，还能描绘具有不同数量表面抗原的细胞亚群。由于FEIA产品既有颜色又有荧光，因此也可以使用简单的光学显微镜来确定标记物在细胞表面的分布。这些技术已应用于检测人红细胞、人T细胞淋巴瘤细胞（H9）上的抗原，以及组织培养细胞系K562上的表面标志物。
