Best L M, Veldhuyzen van Zanten S J, Bezanson G S, Haldane D J, Malatjalian D A
Department of Microbiology, Victoria General Hospital, Halifax, Nova Scotia, Canada.
J Clin Microbiol. 1992 Sep;30(9):2311-7. doi: 10.1128/jcm.30.9.2311-2317.1992.
A flow cytometric immunofluorescence assay (FMIA) for the detection of immunoglobulin G antibodies to Helicobacter pylori was developed. A multicomponent antigen was prepared and used to coat carboxylated polystyrene microspheres for reaction with patient sera followed by fluorescein isothiocyanate-labelled goat anti-human immunoglobulin G. The reacted microspheres were collected with a flow cytometer, and fluorescence was quantitated relative to the cutoff value provided by pooled sera from patients in whom H. pylori could not be demonstrated by culture or histology. Serum samples from 28 H. pylori-positive patients and 27 H. pylori-negative patients were tested by FMIA. Additionally, an in-house enzyme-linked immunosorbent assay (ELISA) employing the same antigen preparation and a commercially available ELISA were used to assay the patient population. Both the FMIA and in-house ELISA were 100% sensitive and 89% specific with positive and negative predictive values of 90 and 100% and no equivocal results. The commercial ELISA was 96% sensitive and 89% specific with positive and negative predictive values of 90 and 96% and five equivocal results. FMIA provides a rapid, inexpensive, and easily performed means for serodiagnosis of H. pylori.
开发了一种用于检测幽门螺杆菌免疫球蛋白G抗体的流式细胞术免疫荧光分析（FMIA）。制备了一种多组分抗原，并将其用于包被羧化聚苯乙烯微球，以与患者血清反应，随后用异硫氰酸荧光素标记的山羊抗人免疫球蛋白G进行反应。用流式细胞仪收集反应后的微球，并相对于来自培养或组织学未证实幽门螺杆菌的患者的混合血清提供的临界值对荧光进行定量。通过FMIA对28例幽门螺杆菌阳性患者和27例幽门螺杆菌阴性患者的血清样本进行了检测。此外，使用相同抗原制剂的内部酶联免疫吸附测定（ELISA）和市售ELISA对患者群体进行检测。FMIA和内部ELISA的敏感性均为100%，特异性为89%，阳性和阴性预测值分别为90%和100%，且无模棱两可的结果。市售ELISA的敏感性为96%，特异性为89%，阳性和阴性预测值分别为90%和96%，并有5个模棱两可的结果。FMIA为幽门螺杆菌的血清学诊断提供了一种快速、廉价且易于操作的方法。
