Biotechnology Program, School of Sciences and Engineering, the American University in Cairo, New Cairo, Egypt.
Department of Biology, School of Sciences and Engineering, the American University in Cairo, New Cairo, Egypt.
Drug Dev Ind Pharm. 2020 Oct;46(10):1613-1621. doi: 10.1080/03639045.2020.1811305. Epub 2020 Aug 28.
Diabetic wound infections and pressure ulcers pose a significant challenge to healthcare providers worldwide. The current study provides new and innovative wound care products that reduce inflammation, clear infection, and improve healing in an animal model of pressure ulcers in diabetic rats. Ointment, hydrogel, and nanofiber dressings were synthesized using 5% turmeric, 1% oregano, and 1% chitosan nanoparticles and tested for antibacterial and cytotoxicity , and wound healing effects . Turmeric ethanolic extract showed high antioxidant activity compared to Oregano, Chitosan Nanoparticles, and Alginate silver (-value < 0.0001). The ointment and hydrogel formulation (5% Turmeric, 1% Oregano, and 1% chitosan) showed lower cytotoxicity compared to the commercial Alginate silver dressing. Ointment, hydrogel formulations, and commercial Alginate silver, showed significant antibacterial activity with 100% efficacy on both and (-value < 0.0001), compared to nanofibers which showed 50% reduction in bacterial growth (-value < 0.0001). The new formulations were tested in a rat model of pressure ulcers. Ointment and nanofibers achieved complete wound healing by day 15 compared to the hydrogel and commercial Alginate silver dressing, which showed higher infection, and the wound remained partially open by day 21. In conclusion, Turmeric, Oregano extracts, and chitosan nanoparticles can be used for effective wound dressings in both diabetic and non-diabetic wounds. At relatively low concentrations, this combination provides a promising new wound treatment formulation that is antibacterial, anti-inflammatory, and antioxidant.
糖尿病性伤口感染和压疮对全球医疗保健提供者构成重大挑战。本研究提供了新的创新伤口护理产品，可在糖尿病大鼠压疮动物模型中减少炎症、清除感染并促进愈合。使用 5%姜黄、1%牛至和 1%壳聚糖纳米颗粒合成了软膏、水凝胶和纳米纤维敷料，并测试了它们的抗菌和细胞毒性以及伤口愈合效果。与牛至、壳聚糖纳米颗粒和藻酸盐银相比，姜黄乙醇提取物显示出更高的抗氧化活性（-值<0.0001）。与商业用藻酸盐银敷料相比，软膏和水凝胶制剂（5%姜黄、1%牛至和 1%壳聚糖）的细胞毒性较低。软膏、水凝胶制剂和商业用藻酸盐银对 和 均显示出 100%的抗菌活性，而纳米纤维对细菌生长的抑制率为 50%（-值<0.0001）。新配方在大鼠压疮模型中进行了测试。与水凝胶和商业用藻酸盐银敷料相比，软膏和纳米纤维在第 15 天实现了完全愈合，而水凝胶和商业用藻酸盐银敷料的感染较高，第 21 天伤口仍部分开放。总之，姜黄、牛至提取物和壳聚糖纳米颗粒可用于治疗糖尿病和非糖尿病伤口的有效伤口敷料。在相对较低的浓度下，这种组合提供了一种有前途的新伤口治疗配方，具有抗菌、抗炎和抗氧化作用。
