Moiseeva T F, Ditiatkin S Ia, Sabel'nikov A G, Il'iashenko B N
Biull Eksp Biol Med. 1977 May;83(5):597-9.
The method of centrifugation in sucrose density gradient (30-55%) of the spheroplast membrane preparations treated and untreated with sturine and infected with phage lambda DNA demonstrated that sturine, treatment increased the phage lambda DNA absorption three-fold. About 50% of the lambda DNA molecules adsorbed by spheroplasts are bound with the cytoplasmic membrane of spheroplasts treated with sturine; 50% of the lambda DNA molecules are bound with the cell wall membrane on the sturine-untreated spheroplasts. The data obtained allow to conclude that the stimulating effect of sturine in E. coli spheroplasts transfection by lambda DNA is connected with redistribution of phage DNA absorbed on spheroplasts from the cell wall to the cytoplasmic membrane facilitating the penetration of DNA and its fastening on the membrane.
在蔗糖密度梯度（30 - 55%）中对经士的宁处理和未处理且感染了λ噬菌体DNA的原生质体膜制剂进行离心的方法表明，士的宁处理使λ噬菌体DNA的吸附增加了三倍。被原生质体吸附的约50%的λDNA分子与经士的宁处理的原生质体的细胞质膜结合；50%的λDNA分子与未用士的宁处理的原生质体的细胞壁膜结合。所获得的数据可以得出结论，士的宁对大肠杆菌原生质体被λDNA转染的刺激作用与吸附在原生质体上的噬菌体DNA从细胞壁重新分布到细胞质膜有关，这有利于DNA的穿透及其在膜上的固定。
