[λ噬菌体分离DNA与大肠杆菌细胞膜结构之间的反应]
[Reaction between isolated lambda phage DNA and membrane structures of Escherichia coli cells].
作者信息
Moiseeva T F, Ditiatkin S Ia, Kim A A, Il'iashenko B N
出版信息
Biull Eksp Biol Med. 1980 Jun;89(6):730-2.
PMID:6446950
Abstract
The saccharose density gradient (30--55%) centrifugation technique applied to E. coli membrane preparations was used to show that treatment of the bacteria with Ca2+ in the cold results in the redistribution of the absorbed phage DNA from the cell wall to the cytoplasmic membrane while freezing-thawing of the bacteria leads to equal distribution of the infectious DNA among all membrane fractions. Quantitative estimation of such a redistribution is reported.
摘要
应用于大肠杆菌膜制剂的蔗糖密度梯度（30%-55%）离心技术表明，在低温下用Ca2+处理细菌会导致吸附的噬菌体DNA从细胞壁重新分布到细胞质膜，而细菌的冻融则导致感染性DNA在所有膜组分中均匀分布。本文报道了这种重新分布的定量估计。
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