Universitat Rovira i Virgili, Departament de Bioquímica i Biotecnologia, Nutrigenomics Research Group, 43007, Tarragona, Spain.
Laboratory of Epigenetics of Lipid Metabolism, Madrid Institute for Advanced Studies (IMDEA)-Food, CEI UAM + CSIC, 28049 Madrid, Spain.
Food Funct. 2020 Sep 23;11(9):7784-7792. doi: 10.1039/d0fo01824d.
(Poly)phenols have varied biological activities that may account for the beneficial effects of fruits and vegetables as part of a healthy diet. Although their cellular absorption and their many mechanisms of action have been partly elucidated, their transport through the systemic circulation, other than their binding to albumin, is poorly described. We aimed at determining whether (poly)phenols can be transported by extracellular vesicles. We supplemented rats with a dietary grape seed polyphenol extract (GSPE) and we quantified (poly)phenols and their metabolites at 3 and 7 h post-gavage. After quantitative LC-MS/MS analysis of circulating aglycones, and microbial-derived, or phase II-derived metabolites we recorded a quantitatively very modest transport of (poly)phenols in plasma exosomes when isolated by commercial ultracentrifugation or precipitation kits. Our data suggest that GSPE-derived (poly)phenols are minimally, if at all, transported by exosomes.
多酚具有多种生物活性，这可能是水果和蔬菜作为健康饮食一部分的有益作用的原因。尽管它们的细胞吸收及其许多作用机制已部分阐明，但除与白蛋白结合外，它们在全身循环中的转运情况描述甚少。我们旨在确定多酚是否可以通过细胞外囊泡进行转运。我们用饮食补充葡萄籽多酚提取物（GSPE）喂养大鼠，并在灌胃后 3 和 7 小时定量检测多酚及其代谢物。在对循环糖苷配基、微生物衍生或 II 相衍生代谢物进行定量 LC-MS/MS 分析后，我们发现通过商业超速离心或沉淀试剂盒分离的血浆外泌体中（聚）多酚的转运量非常少。我们的数据表明，GSPE 衍生的（聚）多酚很少（如果有的话）通过外泌体转运。
