Chen Ya Wei, Liao Yuan, Kong Wei Zhen, Wang Shu Han
College of Chemical and Pharmaceutical Engineering, Henan University of Science and Technology, 263 Kaiyuan Avenue, Luoyang, 471023, People's Republic of China.
Biotechnol Lett. 2020 Dec;42(12):2581-2587. doi: 10.1007/s10529-020-02989-9. Epub 2020 Aug 17.
In general, a sufficient supply of ATP can promote the synthesis of ATP-driven metabolites, but excessive ATP will lead to the inhibition of cell growth. For enhancing the co-production of glutathione(GSH) and S-adenosylmethionine(SAM), a dynamic ATP regeneration strategy was developed.
The novel ATP regeneration strategy consisting of ATP-sensing riboswitch ydaO motif, polyphosphate kinase (PPK), and Vitreoscilla hemoglobin (VHb) was successfully applied in Escherichia coli. The intracellular ATP level was always around 0.60 mg/g dry cell weight during the fermentation process, resulting in significantly enhanced co-production of GSH and SAM. The GSH titer and SAM titer in the strain CGS-2 increased by 137.40% and 82.18% after fermentation for 24 h, compared with the control strain.
The ATP regulation strategy is expected to be a favorable tool to improve the efficiency of microbial cell factories. The proposed ATP dynamic regeneration approach may be applicable for cost-effective, high-yield production of ATP-driven metabolites.
一般来说，充足的ATP供应可促进ATP驱动的代谢产物合成，但过量的ATP会导致细胞生长受到抑制。为提高谷胱甘肽（GSH）和S-腺苷甲硫氨酸（SAM）的联产，开发了一种动态ATP再生策略。
由ATP感应核糖开关ydaO基序、多聚磷酸激酶（PPK）和透明颤菌血红蛋白（VHb）组成的新型ATP再生策略成功应用于大肠杆菌。发酵过程中细胞内ATP水平始终保持在0.60 mg/g干细胞重左右，从而显著提高了GSH和SAM的联产。与对照菌株相比，菌株CGS-2发酵24 h后GSH产量和SAM产量分别提高了137.40%和82.18%。
ATP调控策略有望成为提高微生物细胞工厂效率的有利工具。所提出的ATP动态再生方法可能适用于ATP驱动的代谢产物的经济高效、高产生产。
