比较血清和肺提取物用于野生动物中针对土拉热弗朗西斯菌古北区生物变种抗体的检测。

Comparison of serum and lung extracts for surveys of wild animals for antibodies to Francisella tularensis biovar palaearctica.

作者信息

Mörner T, Sandström G, Mattsson R

机构信息

Department of Wildlife Diseases, National Veterinary Institute, Uppsala, Sweden.

出版信息

J Wildl Dis. 1988 Jan;24(1):10-4. doi: 10.7589/0090-3558-24.1.10.

DOI:10.7589/0090-3558-24.1.10

PMID:3280838

Abstract

A comparative study of the antibody titer against Francisella tularensis biovar palaearctica in serum and lung extract was made using different immunoassays. Samples were taken from experimentally-infected goshawks (Accipiter gentilis) and European beavers (Castor fiber), and in a field survey of wild beavers. Good accordance between the antibody titer in serum and in lung extract was found in the experimental studies. However, the antibody titer was generally one- to three-fold lower in lung extract than was the titer of serum. Results in the field survey were less reliable. Estimating antibody titer in lung extract is a practical method for surveys for antibody levels, and an alternative to serological surveys, despite lower sensitivity as compared to serum.

摘要

利用不同的免疫测定方法，对血清和肺提取物中针对土拉热弗朗西斯菌古北区生物变种的抗体滴度进行了比较研究。样本取自实验感染的苍鹰（Accipiter gentilis）和欧洲河狸（Castor fiber），以及对野生河狸的实地调查。在实验研究中发现血清和肺提取物中的抗体滴度具有良好的一致性。然而，肺提取物中的抗体滴度通常比血清滴度低一至三倍。实地调查的结果不太可靠。尽管与血清相比灵敏度较低，但估计肺提取物中的抗体滴度是一种检测抗体水平的实用方法，也是血清学检测的一种替代方法。