Institute of Biomaterial Science and Berlin-Brandenburg Centre for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, Teltow, 14513, Germany.
Institute of Chemistry, University of Potsdam, Potsdam, 14476, Germany.
Macromol Biosci. 2020 Oct;20(10):e2000221. doi: 10.1002/mabi.202000221. Epub 2020 Aug 17.
Hydrogel forming physical networks based on gelatin are an attractive approach toward multifunctional biomaterials with the option of reshaping, self-healing, and stimuli-sensitivity. However, it is challenging to design such gelatin-based hydrogels to be stable at body temperature. Here, gelatin functionalized with desaminotyrosine (DAT) or desaminotyrosyl tyrosine (DATT) side chains is crosslinked with cyclodextrin (CD) dimers under formation of inclusions complexes. The supramolecular networks displayed at room temperature decreased water uptake (200-600 wt% for DAT-based systems, 200 wt% for DATT based systems), and increased storage moduli up to 25.6 kPa determined by rheology compared to DAT(T) gelatin. The gel-sol transition temperature increased from 33 up to 42 °C. The presented system that is completely based on natural building blocks may form the basis for materials that may potentially respond by dissolution or changes of properties to changes in environmental conditions or to the presence of CD guest molecules.
基于明胶的水凝胶形成物理网络是一种很有吸引力的方法，可以制备多功能生物材料，具有可重塑、自修复和刺激响应的特性。然而，设计在体温下稳定的基于明胶的水凝胶具有挑战性。在这里，用去氨基酪氨酸（DAT）或去氨基酪氨酸基酪氨酸（DATT）侧链修饰的明胶与环糊精（CD）二聚体交联，形成包合物复合物。在室温下，超分子网络的水吸收减少（基于 DAT 的系统为 200-600wt%，基于 DATT 的系统为 200wt%），流变学测定的储能模量增加到 25.6kPa，与 DAT（T）明胶相比有所提高。凝胶-溶胶转变温度从 33°C 升高到 42°C。该系统完全基于天然构建块，可能为材料奠定基础，这些材料可能会通过溶解或改变性质来响应环境条件的变化或 CD 客体分子的存在。
