DOE Joint Genome Institute (JGI), Berkeley, CA, USA.
Environmental Genomics & Systems Biology, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Methods Mol Biol. 2020;2205:3-18. doi: 10.1007/978-1-0716-0908-8_1.
Biological computer-aided design and manufacturing (bioCAD/CAM) tools facilitate the design and build processes of engineering biological systems using iterative design-build-test-learn (DBTL) cycles. In this book chapter, we highlight some of the bioCAD/CAM tools developed and used at the US Department of Energy (DOE) Joint Genome Institute (JGI), Joint BioEnergy Institute (JBEI), and Agile BioFoundry (ABF). We demonstrate the use of these bioCAD/CAM tools on a common workflow for designing and building a multigene pathway in a hierarchical fashion. Each tool presented in this book chapter is specifically tailored to support one or more specific steps in a workflow, can be integrated with the others into design and build workflows, and can be deployed at academic, government, or commercial entities.
生物计算机辅助设计和制造 (bioCAD/CAM) 工具通过迭代设计-构建-测试-学习 (DBTL) 循环，促进工程生物系统的设计和构建过程。在本章中，我们重点介绍了美国能源部 (DOE) 联合基因组研究所 (JGI)、联合生物能源研究所 (JBEI) 和敏捷生物制造工厂 (ABF) 开发和使用的一些 bioCAD/CAM 工具。我们展示了如何在一个常见的工作流程中使用这些 bioCAD/CAM 工具，以层次化的方式设计和构建多基因途径。本章中介绍的每个工具都专门针对一个或多个工作流程中的特定步骤进行了定制，可以与其他工具集成到设计和构建工作流程中，并可以部署在学术、政府或商业实体中。
