在联合基因组研究所构建用于DNA构建体设计与组装的定制高通量平台——当前与未来的挑战
Building a custom high-throughput platform at the Joint Genome Institute for DNA construct design and assembly-present and future challenges.
作者信息
Blaby Ian K, Cheng Jan-Fang
机构信息
US Department of Energy, Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
出版信息
Synth Biol (Oxf). 2020 Nov 5;5(1):ysaa023. doi: 10.1093/synbio/ysaa023. eCollection 2020.
Abstract
The rapid design and assembly of synthetic DNA constructs have become a crucial component of biological engineering projects via iterative design-build-test-learn cycles. In this perspective, we provide an overview of the workflows used to generate the thousands of constructs and libraries produced each year at the U.S. Department of Energy Joint Genome Institute. Particular attention is paid to describing pipelines, tools used, types of scientific projects enabled by the platform and challenges faced in further scaling output.
摘要
通过迭代式设计-构建-测试-学习循环，合成DNA构建体的快速设计与组装已成为生物工程项目的关键组成部分。从这个角度出发，我们概述了美国能源部联合基因组研究所每年用于生成数千个构建体和文库的工作流程。特别关注描述流程、所使用的工具、该平台支持的科学项目类型以及在进一步扩大产出时面临的挑战。
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