酶联免疫吸附法与免疫荧光法在HIV感染血清学诊断中的比较。
Comparison between enzyme-linked immunosorbent and I.F. assays in the serological diagnosis of HIV infections.
作者信息
Borghi V, De Palma M, Vecchi M, Pellegrino F, Mongiardo N, Cocchi I, De Rienzo B, Squadrini F
机构信息
Department of Infectious Diseases, University of Modena, Italy.
出版信息
Microbiologica. 1988 Jan;11(1):81-4.
A rapid, sensitive indirect immunofluorescence assay based on the use of acetone-fixed virus producing MOLT T4/LAV cells was adapted for the detection of anti-HIV antibodies. At the same time, 1486 serum samples, collected by the AIDS Surveillance and Study Centre of Modena, were tested by ELISA and IFA: the percentage of agreement of both assays was of 99.66%. Such datum suggested that IFA could be used as a serological assay for screening and confirmatory purposes.
基于使用丙酮固定的产生病毒的MOLT T4/LAV细胞，建立了一种快速、灵敏的间接免疫荧光测定法用于检测抗HIV抗体。同时，对摩德纳艾滋病监测与研究中心收集的1486份血清样本进行了酶联免疫吸附测定（ELISA）和免疫荧光测定（IFA）：两种检测方法的符合率为99.66%。这一数据表明，免疫荧光测定法可作为一种用于筛查和确证目的的血清学检测方法。
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