Tudek B, Gajewska J, Szczypka M, Rahden-Staron I, Szymczyk T
Department of Biochemistry, Medical School, Warsaw, Poland.
Mutat Res. 1988 Apr;204(4):585-91. doi: 10.1016/0165-1218(88)90061-4.
Genotoxic effects of both amitraz and its metabolites made by S9 fraction were reevaluated in short-term bacterial assays. Neither amitraz nor its metabolites induced frameshift mutation or caused base-pair substitution as detected by the Ames test. They also did not introduce any damages into DNA recognized by correndonuclease II as shown by the repair test. Metabolites of amitraz (but not amitraz itself) induced the SOS-repair system in E. coli strain PQ 243 tagA, alkA which was deficient in N-glycosylases. It is concluded that neither amitraz nor its metabolites have mutagenic activity. In contrast to amitraz, its metabolites alkylate DNA in the N3-position of adenine.
通过短期细菌试验对双甲脒及其经S9组分代谢产生的代谢产物的遗传毒性作用进行了重新评估。如Ames试验所检测，双甲脒及其代谢产物均未诱导移码突变或引起碱基对置换。修复试验表明，它们也未对核酸外切酶II识别的DNA造成任何损伤。双甲脒的代谢产物（而非双甲脒本身）在缺乏N-糖苷酶的大肠杆菌菌株PQ 243 tagA、alkA中诱导了SOS修复系统。得出的结论是，双甲脒及其代谢产物均无诱变活性。与双甲脒不同，其代谢产物使腺嘌呤的N3位DNA发生烷基化。
