Ruiz M J, Marzin D
Laboratori de Toxicologia, Facultat de Formàcia, Universitat de València, Spain.
Mutat Res. 1997 May 23;390(3):245-55. doi: 10.1016/s1383-5718(97)00021-1.
Two in vitro tests (Ames test and SOS chromotest), one for bacterial mutagenicity and one for primary DNA damage, were assayed to determine the genotoxic activity of 6 pesticides (atrazine, captafol, Captan, chlorpyrifosmethyl, molinate and tetrachlorvinphos). Assays were carried out both in the absence and presence of S9 fractions of liver homogenate from rat (Sprague-Dawley) pretreated with Aroclor 1254. Captan and captafol were genotoxic on both the Ames test and the SOS chromotest. Comparisons with mutagenesis data in Salmonella indicated that the SOS assay detected as genotoxic the pesticides that were mutagenic on the Salmonella test. Non-genotoxic effects were not detected in vitro either in the Salmonella/microsome assay nor in the SOS chromotest when bacterial tester strains were exposed to atrazine, molinate, chlorpyrifosmethyl and tetrachlorvinphos in the absence or presence of S9 mix.
进行了两项体外试验（艾姆斯试验和SOS显色试验），一项用于检测细菌致突变性，另一项用于检测原发性DNA损伤，以确定6种农药（莠去津、克菌丹、福美双、甲基毒死蜱、禾草敌和杀虫畏）的遗传毒性活性。试验在不存在和存在经Aroclor 1254预处理的大鼠（斯普拉格-道利）肝脏匀浆的S9组分的情况下进行。福美双和克菌丹在艾姆斯试验和SOS显色试验中均具有遗传毒性。与沙门氏菌中的诱变数据比较表明，SOS试验检测出在沙门氏菌试验中具有诱变性的农药具有遗传毒性。当细菌试验菌株在不存在或存在S9混合物的情况下暴露于莠去津、禾草敌、甲基毒死蜱和杀虫畏时，在沙门氏菌/微粒体试验或SOS显色试验中均未检测到非遗传毒性效应。
