Fibrosis Research Group, Institute of Cellular Medicine, Newcastle University, United Kingdom.
Bioinformatics Support Unit, Faculty of Medical Sciences, Newcastle University, United Kingdom.
Rhinology. 2020 Dec 1;58(6):581-587. doi: 10.4193/Rhin19.319.
Chronic rhinosinusitis is an inflammatory condition with an as yet unknown pathophysiology. We aimed to detect clusters of differentially regulated genes in the epithelial and fibroblast cells of patients with Chronic Rhinosinusitis without nasal polyposis (CRSsNP) and healthy controls.
Carefully phenotyped CRSsNP and healthy control participants were recruited. Primary cultures of isolated epithelial and fibroblast cells were established. Whole transcriptome analysis of the cells was performed using microarrays and replicated with quantitative RT-PCR and immunohistochemistry.
Fibroblast cells from CRSsNP patients showed a significant upregulation (more than 2x) of the transcription factor NFE2L3 when compared to healthy controls by microarray with multiple hypothesis testing correction, qRT-PCR and immunohistochemistry.
Here we have utilized microarray analysis to search for differentially expressed genes in isolated patient derived epithelial and fibroblast cells. The transcription factor NFE2L3 has been shown to be upregulated in fibroblast cells consistent with increasing evidence that fibroblasts play a key role in tissue specific inflammation within the paranasal sinuses.
慢性鼻-鼻窦炎是一种炎症性疾病，其病理生理学尚不清楚。我们旨在检测无鼻息肉的慢性鼻-鼻窦炎（CRSsNP）患者和健康对照者的上皮细胞和成纤维细胞中差异调节基因的簇。
我们招募了精心表型的 CRSsNP 和健康对照者。建立了分离的上皮细胞和成纤维细胞的原代培养物。使用微阵列对细胞进行全转录组分析，并通过定量 RT-PCR 和免疫组织化学进行了复制。
与健康对照组相比，通过微阵列进行多重假设检验校正、qRT-PCR 和免疫组织化学分析，CRSsNP 患者的成纤维细胞中转录因子 NFE2L3 的表达显著上调（超过 2 倍）。
在这里，我们利用微阵列分析来搜索分离的患者来源的上皮细胞和成纤维细胞中差异表达的基因。转录因子 NFE2L3 在成纤维细胞中上调，这与越来越多的证据一致，即成纤维细胞在鼻窦组织特异性炎症中起关键作用。
