Mine Yuichi, Suga Mika, Mimura Sumiyo, Minoda Memori, Murayama Takeshi, Nikawa Hiroki, Furue Miho K
Department of Medical System Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
Translational Research Center, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima, 734-8553, Japan.
In Vitro Cell Dev Biol Anim. 2020 Aug;56(7):505-510. doi: 10.1007/s11626-020-00491-0. Epub 2020 Aug 18.
Cleft lip and palate are the most common congenital abnormalities that occur early in pregnancy. The majority of cranial mesenchyme is derived from cranial neural crest cells that differentiate into odontoblasts, cartilage, craniofacial bone, and connective tissue. A subset of these cells differentiates into cranial ganglia. We have previously reported an induction protocol of cranial neural crest cell-like cells from human pluripotent stem cells. This study tested detection of the cytotoxic sensitivities of dental materials, including titanium ions, palladium ions, and hydroxyethyl methacrylate, on the cell viability of induced cranial neural crest cell-like cells (iNC-LCs) derived from Tic human induced pluripotent stem cell (hiPSC) line. Further, the sensitivity was compared with those of human fetal lung fibroblastic cell line MRC-5, which is origin of Tic hiPSC, and osteoblastic cell line MC3T3-E1 which was derived from mouse calvaria. The results suggested that this cell-based assay system using iNC-LCs is a potential method for in vitro screening as an alternative to animal testing to predict toxic effects of dental materials on early craniofacial development.
唇腭裂是孕期早期最常见的先天性畸形。大多数颅间充质来源于颅神经嵴细胞，这些细胞可分化为成牙本质细胞、软骨、颅面骨和结缔组织。其中一部分细胞会分化为颅神经节。我们之前报道了一种从人多能干细胞诱导生成颅神经嵴样细胞的方法。本研究检测了包括钛离子、钯离子和甲基丙烯酸羟乙酯在内的牙科材料对源自Tic人诱导多能干细胞（hiPSC）系的诱导颅神经嵴样细胞（iNC-LCs）细胞活力的细胞毒性敏感性。此外，还将该敏感性与Tic hiPSC的起源细胞——人胎儿肺成纤维细胞系MRC-5以及源自小鼠颅骨的成骨细胞系MC3T3-E1的敏感性进行了比较。结果表明，这种使用iNC-LCs的基于细胞的检测系统是一种潜在的体外筛选方法，可替代动物试验来预测牙科材料对早期颅面发育的毒性作用。
