Suga Mika, Furue Miho K
Laboratory of Stem Cell Cultures, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Ibaraki, Japan.
Methods Mol Biol. 2019;1965:35-48. doi: 10.1007/978-1-4939-9182-2_4.
Cranial neural crest cells (NCCs) migrate to the branchial arches and give rise to the majority of cranial mesenchyme that eventually differentiates into odontoblasts, cartilage, craniofacial bone, and connective tissue; a subset of these cells differentiate into cranial ganglia. Here we present a protocol that describes directed differentiation method of human pluripotent stem cells into cranial NCC-like cells and a cytotoxicity assay using hPSC-derived cranial NCC-like cells. This cell-based assay system allows for high-sensitive cytotoxicity detection of test chemicals. These methods can be applied to predict drug/chemical toxicity effect on early craniofacial development.
颅神经嵴细胞（NCCs）迁移至鳃弓，并产生大部分颅间充质，这些颅间充质最终分化为成牙本质细胞、软骨、颅面骨和结缔组织；这些细胞的一个亚群分化为颅神经节。在此，我们介绍一种方案，该方案描述了将人多能干细胞定向分化为颅神经嵴样细胞的方法，以及使用人多能干细胞衍生的颅神经嵴样细胞进行细胞毒性测定。这种基于细胞的检测系统能够对测试化学品进行高灵敏度的细胞毒性检测。这些方法可用于预测药物/化学品对早期颅面发育的毒性作用。
