新型可生物降解植入系统对大鼠胫骨骨折模型的影响。

Effects of a novel biodegredable implant system on a rat tibia fracture model.

机构信息

Department of Orthopaedics and Traumatology, Near East University Hospital, Mersin, Turkey.

Department of Orthopaedics and Traumatology, Sakarya University School of Medicine, Sakarya, Turkey.

出版信息

Acta Orthop Traumatol Turc. 2020 Jul;54(4):453-460. doi: 10.5152/j.aott.2020.18331.

DOI:10.5152/j.aott.2020.18331

PMID:32812878

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7444870/

Abstract

OBJECTIVE

This study aimed to determine the effects of a novel biodegradable implant releasing platelet-derived growth factor (PDGF) at the fracture site on fracture healing in a rat tibia fracture model.

METHODS

In this study, 35 male Sprague-Dawley rats weighing between 300 and 350 g were used. The rats were divided into four groups: Group A (control group without any treatment, n=10), Group B (spacer without PDGF Group, n=10), Group C (spacer with PDGF group, n=10), and Group D (healthy rat Group, n=5). Standardized fractures were created in the right tibias of rats, and then biodegradable implants made of poly-β-hydroxybutyrate-co-3-hydroxy valerate were implanted at the fracture sites in Groups B and C. In Group C, implants were loaded with 600 ng of PDGF. Animals were sacrificed 30 days after the operation, and fracture healing in each group was assessed radiologically based on the Goldberg score. Furthermore, the anteroposterior (AP) and mediolateral (ML) callus diameters were measured macroscopically, and fracture sites were mechanically tested.

RESULTS

In the radiological assessment, Group C showed higher fracture healing rate than Groups A and B (p=0.001), whereas no significant difference was found between group C and Group D (p>0.05). In the macroscopic assessment, while Group C exhibited the thickest AP callus diameter (p=0.02), no significant differences in ML callus diameters existed among the groups (p>0.05). Mechanical testing revealed that Group C had higher torsional strength (p=0.001) and stiffness than Groups A and B (p=0.001) while there was no significant difference between Groups C and D (p>0.05).

CONCLUSION

Biodegradable implant releasing PDGF may have positive effects on fracture healing.

摘要

目的

本研究旨在探讨一种新型可生物降解的局部释放血小板衍生生长因子（PDGF）的植入物对大鼠胫骨骨折模型骨折愈合的影响。

方法

本研究使用 35 只体重 300-350g 的雄性 Sprague-Dawley 大鼠。将大鼠分为四组：A 组（无任何治疗的对照组，n=10）、B 组（无 PDGF 的间隔物组，n=10）、C 组（含 PDGF 的间隔物组，n=10）和 D 组（健康大鼠组，n=5）。在大鼠右侧胫骨上制造标准化骨折，然后在 B 组和 C 组的骨折部位植入由聚β-羟基丁酸-co-3-羟基戊酸制成的可生物降解植入物。在 C 组中，植入物加载 600ng PDGF。术后 30 天处死动物，根据 Goldberg 评分对每组的骨折愈合情况进行影像学评估。此外，通过宏观测量前-后（AP）和内-外侧（ML）骨痂直径，并进行骨折部位的力学测试。

结果

在影像学评估中，C 组的骨折愈合率高于 A 组和 B 组（p=0.001），而 C 组与 D 组之间无显著差异（p>0.05）。在宏观评估中，C 组的 AP 骨痂直径最厚（p=0.02），但各组之间的 ML 骨痂直径无显著差异（p>0.05）。力学测试显示，C 组的扭转强度（p=0.001）和刚度均高于 A 组和 B 组（p=0.001），而 C 组与 D 组之间无显著差异（p>0.05）。