Chen Xinyu, Jia Wei, Wang Qiao, Han Jianxin, Cheng Jun, Zeng Weijiang, Zhao Qingning, Zhang Yu, Zhang Ying
Zhejiang Key Laboratory for Agro-Food Processing, Key Laboratory of Agro-Products Postharvest Handling of Ministry of Agriculture and Rural Affairs, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, China.
Food Funct. 2020 Aug 1;11(8):7000-7011. doi: 10.1039/d0fo00811g. Epub 2020 Jul 24.
Polyphenolic antioxidants may effectively reduce acrylamide contents in processed foods. However, few studies focused on their detoxification effects via estimating the profile change of internal exposure biomarkers. Here we showed the protective effect of a water-soluble flavone-C-glycoside-rich antioxidant from bamboo leaves (AOB-w) against acrylamide-induced toxicity in college students. The participants were randomly assigned to either the AOB-w or control group and served potato chips, corresponding to 12.6 μg per kg·bw of dietary exposure to acrylamide, followed by capsules containing 350 mg AOB-w or equivalent placebo. The kinetics of acrylamide, glycidamide, and mercapturic acid metabolites was profiled, and their hemoglobin adducts were measured. The toxicokinetic study showed that AOB-w promoted the excretion of acrylamide and shortened the distribution but prolonged the excretion of N-acetyl-S-(2-carbamoylethyl)-l-cysteine (AAMA) and N-acetyl-S-(2-carbamoyl-2-hydroxyethyl)-l-cysteine. The intervention with AOB-w reduced the peak concentration and area under curve of AAMA by 42.1% and 49.8%, respectively. Besides, AOB-w gender-dependently altered the toxicokinetic profile and reduced the amount of a human-specific urinary biomarker, N-acetyl-S-(2-carbamoylethyl)-l-cysteine-sulfoxide in women. AOB-w accelerated the metabolism of hemoglobin adducts of acrylamide and glycidamide in blood of women. Compared with the baseline levels on the beginning day, we observed a significant enhancement of hemoglobin adducts on the 10th day after serving them potato chips, showing 54.5% and 20.9% higher levels of the hemoglobin adducts of acrylamide and glycidamide, respectively, which thus indicated a lower level of glycidamide-to-acrylamide ratio in blood of participants. Overall AOB-w could effectively reduce the internal exposure to acrylamide in college students, which provides advanced insights into protective functions of natural antioxidants against in vivo toxicity of chemical contaminants from diet.
多酚类抗氧化剂可能有效降低加工食品中的丙烯酰胺含量。然而，很少有研究通过评估体内暴露生物标志物的谱变化来关注它们的解毒作用。在此，我们展示了一种富含水溶性黄酮碳苷的竹叶抗氧化剂（AOB-w）对大学生丙烯酰胺诱导毒性的保护作用。参与者被随机分为AOB-w组或对照组，食用薯片，相当于每千克体重摄入12.6微克的膳食丙烯酰胺暴露量，随后服用含有350毫克AOB-w或等效安慰剂的胶囊。分析了丙烯酰胺、缩水甘油酰胺和硫醚氨酸代谢物的动力学，并测量了它们的血红蛋白加合物。毒代动力学研究表明，AOB-w促进了丙烯酰胺的排泄，缩短了分布时间，但延长了N-乙酰-S-(2-氨甲酰基乙基)-L-半胱氨酸（AAMA）和N-乙酰-S-(2-氨甲酰基-2-羟乙基)-L-半胱氨酸的排泄时间。AOB-w干预分别使AAMA的峰值浓度和曲线下面积降低了42.1%和49.8%。此外，AOB-w对毒代动力学谱的影响存在性别差异，并降低了女性体内一种人类特异性尿液生物标志物N-乙酰-S-(2-氨甲酰基乙基)-L-半胱氨酸亚砜的含量。AOB-w加速了女性血液中丙烯酰胺和缩水甘油酰胺血红蛋白加合物的代谢。与食用薯片当天的基线水平相比，我们观察到在食用薯片后第10天血红蛋白加合物显著增加，丙烯酰胺和缩水甘油酰胺的血红蛋白加合物水平分别高出54.5%和20.9%，这表明参与者血液中缩水甘油酰胺与丙烯酰胺的比例较低。总体而言，AOB-w可以有效降低大学生体内丙烯酰胺的暴露量，这为天然抗氧化剂对饮食中化学污染物体内毒性的保护作用提供了新的见解。
