Yildirim Metin, Degirmenci Ulas, Akkapulu Merih, Comelekoglu Ulku, Balli Ebru, Metin Ozcan Tuba, Berköz Mehmet, Yalin Ali Erdinc, Yalin Serap
Department of Biochemistry, Faculty of Pharmacy, Mersin University, Mersin, Turkey.
Department of Biophysics, Faculty of Medicine, Mersin University, Mersin, Turkey.
J Basic Clin Physiol Pharmacol. 2020 Aug 15. doi: 10.1515/jbcpp-2020-0058.
Objectives Rheum ribes L. is a perennial plant that belongs to the family of Polygonaceae, which is often used in traditional therapy because it possesses many bioactivities, such as antioxidant and antibacterial ones. Here we examined the effect of different R. ribes L. extracts on oxidative stress in experimental diabetic rats. Methods Thirty-six rats were divided into six groups as follows: group I, control group; group II, diabetic rats; group III, diabetic rats treated with the aqueous extract of R. ribes L. by gavage at 50 mg/kg for 15 days; group IV, diabetic rats treated by gavage with the ethanolic extract of R. ribes L. at 50 mg/kg for 15 days; group V, nondiabetic rats treated by gavage with the aqueous extract of R. ribes L. at 50 mg/kg for 15 days; group VI, nondiabetic rats treated by gavage with the ethanol extract of R. ribes L. at 50 mg/kg for 15 days. After 15 days, the animals were sacrificed and the liver and kidney tissues of each animal were isolated. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) activities in the tissue samples were measured, and histopathologic examination was carried out. Results R. ribes L. was effective in reducing the oxidative stress and increasing the levels of the antioxidant enzymes. Increased levels of MDA and decreased levels of SOD, CAT and GSH-Px were observed in both the liver and kidney tissues in group II. Decreased levels of MDA and increased levels of SOD, CAT and GSH-Px were observed in group III compared with group II. In group IV, decreased levels of MDA and increased levels of SOD, CAT and GSH-Px were observed in comparison with group II. Conclusions Diabetes increases oxidative stress and causes a decrease in antioxidant enzyme levels. Both aqueous and ethanolic extracts of R. ribes L. decrease oxidative stress activity and increase the levels of antioxidant enzymes. The ethanol extract of R. ribes L. has a higher antioxidant effect than the aqueous extract.
目的 黑果枸杞是一种多年生植物，属于蓼科，因其具有许多生物活性，如抗氧化和抗菌活性，常被用于传统疗法。在此，我们研究了不同黑果枸杞提取物对实验性糖尿病大鼠氧化应激的影响。方法 将36只大鼠分为以下六组：第一组，对照组；第二组，糖尿病大鼠组；第三组，糖尿病大鼠经口灌胃给予50mg/kg黑果枸杞水提取物，持续15天；第四组，糖尿病大鼠经口灌胃给予50mg/kg黑果枸杞乙醇提取物，持续15天；第五组，非糖尿病大鼠经口灌胃给予50mg/kg黑果枸杞水提取物，持续15天；第六组，非糖尿病大鼠经口灌胃给予50mg/kg黑果枸杞乙醇提取物，持续15天。15天后，处死动物，分离每只动物的肝脏和肾脏组织。测定组织样本中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）的活性，并进行组织病理学检查。结果 黑果枸杞在减轻氧化应激和提高抗氧化酶水平方面有效。第二组肝脏和肾脏组织中MDA水平升高，SOD、CAT和GSH-Px水平降低。与第二组相比，第三组MDA水平降低，SOD、CAT和GSH-Px水平升高。与第二组相比时，第四组MDA水平降低，SOD、CAT和GSH-Px水平升高。结论 糖尿病会增加氧化应激并导致抗氧化酶水平降低。黑果枸杞的水提取物和乙醇提取物均能降低氧化应激活性并提高抗氧化酶水平。黑果枸杞的乙醇提取物比水提取物具有更高的抗氧化作用。
