Gholamnezhad Zahra, Hadjzadeh Mousa-Al-Reza, Sotoudeh Reyhaneh, Aghaei Azita, Kasraie Narges
Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Vet Res Forum. 2021 Dec;12(4):459-465. doi: 10.30466/vrf.2020.113744.2707. Epub 2021 Dec 15.
The anti-diabetic effects of as a traditional remedy were investigated in diabetic rats. Thirty-five male rats were divided into five groups: control, diabetic, diabetic treated with metformin (300 mg kg; D+Met), diabetic treated with 250 and 500 mg kg of hydro-ethanolic extract (D+Rib250 and D+Rib500). After six weeks of treatment, sera of overnight fasted animals were collected and used for measurement of glucose, insulin, lipid profile, urea, creatinine, and hepatic enzymes levels. Moreover, liver and kidney of rats were removed and used for measurement of oxidative stress including malondialdehyde (MDA), thiol content, and the activity of catalase (CAT) and superoxide dismutase (SOD). Streptozotocin (STZ)-induced diabetes increased the levels of serum glucose, triglycerides (TG), total cholesterol (TC), and LDL-C, urea, creatinine, hepatic enzymes, and kidney and liver oxidative stress markers, while decreased insulin and HDL-C when compared to control group. In all treated groups serum levels of glucose, TC, LDL-C, TG, and urea were decreased, while liver SOD activity was increased compared to the diabetic group. The D+Rib500 group had lower Serum glutamic pyruvic transaminase (SGPT), creatinine, and kidney MDA levels, but higher insulin, HDL-C levels, liver CAT activity, and kidney thiol content, and CAT activity compared to diabetic group. In D+Met group, serum levels of serum glutamic-oxaloacetic transaminase (SGOT), creatinine, and MDA of liver and kidney were decreased, while liver SOD activity was increased compared to the diabetic group. Based on our findings, treatment with improved diabetic complications, while the effect of a higher dose of the extract was comparable to metformin's.
研究了一种传统药物的抗糖尿病作用，该研究以糖尿病大鼠为对象。35只雄性大鼠被分为五组：对照组、糖尿病组、用二甲双胍（300 mg/kg；糖尿病 + 二甲双胍组）治疗的糖尿病组、用250 mg/kg和500 mg/kg的水乙醇提取物治疗的糖尿病组（糖尿病 + 核黄素250组和糖尿病 + 核黄素500组）。治疗六周后，收集过夜禁食动物的血清，用于测量血糖、胰岛素、血脂谱、尿素、肌酐和肝酶水平。此外，取出大鼠的肝脏和肾脏，用于测量氧化应激指标，包括丙二醛（MDA）、巯基含量以及过氧化氢酶（CAT）和超氧化物歧化酶（SOD）的活性。与对照组相比，链脲佐菌素（STZ）诱导的糖尿病增加了血清葡萄糖、甘油三酯（TG）、总胆固醇（TC）和低密度脂蛋白胆固醇（LDL-C）、尿素、肌酐、肝酶以及肾脏和肝脏氧化应激标志物的水平，同时降低了胰岛素和高密度脂蛋白胆固醇（HDL-C）水平。与糖尿病组相比，所有治疗组的血清葡萄糖、TC、LDL-C、TG和尿素水平均降低，而肝脏SOD活性增加。与糖尿病组相比，糖尿病 + 核黄素500组的血清谷丙转氨酶（SGPT）、肌酐和肾脏MDA水平较低，但胰岛素、HDL-C水平、肝脏CAT活性、肾脏巯基含量和CAT活性较高。在糖尿病 + 二甲双胍组中，血清谷草转氨酶（SGOT）、肌酐以及肝脏和肾脏的MDA水平降低，而与糖尿病组相比，肝脏SOD活性增加。基于我们的研究结果，用该药物治疗可改善糖尿病并发症，且较高剂量提取物的效果与二甲双胍相当。
