Clarke S M, Fink L M
Cancer Res. 1977 Sep;37(9):2985-92.
Antibodies were produced against 220,000-molecular-weight proteins of Syrian hamster embryo cells. The antiserum containing these antibodies is capable of immunologically staining the surfaces and a fibrillar network around untransformed Syrian hamster embryo cells. The staining is removed by adsorption of the antiserum with Syrian hamster embryo cells and by mild trypsin treatment. Several lines of neoplastically transformed Syrian hamster embryo cells isolated and cloned after treatment with chemical carcinogens show little or no immune staining. Adsorption of the antiserum with certain transformed cells does not significantly reduce the immune staining of untransformed Syrian hamster embryo cells. The immune antiserum, in the presence of complement, is selectively cytotoxic to the untransformed Syrian hamster embryo cells. However, the transformed lines show resistance to this treatment. Analysis of the lactoperoxidase-catalyzed, iodinated cell surface proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis reveals that the transformed lines have either no detectable labeling or a marked reduction in the labeling of 220,000-molecular-weight proteins that are major iodinatable cell surface proteins on untransformed Syrian hamster embryo cells.
制备了针对叙利亚仓鼠胚胎细胞220,000分子量蛋白质的抗体。含有这些抗体的抗血清能够对未转化的叙利亚仓鼠胚胎细胞的表面和周围的纤维状网络进行免疫染色。通过用叙利亚仓鼠胚胎细胞吸附抗血清以及用温和的胰蛋白酶处理可以去除染色。在用化学致癌物处理后分离和克隆的几株肿瘤转化的叙利亚仓鼠胚胎细胞几乎没有或没有免疫染色。用某些转化细胞吸附抗血清不会显著降低未转化的叙利亚仓鼠胚胎细胞的免疫染色。免疫抗血清在补体存在下对未转化的叙利亚仓鼠胚胎细胞具有选择性细胞毒性。然而，转化细胞系对这种处理具有抗性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析乳过氧化物酶催化的碘化细胞表面蛋白表明，转化细胞系要么没有可检测到的标记，要么在220,000分子量蛋白质的标记上有显著减少，而这些蛋白质是未转化的叙利亚仓鼠胚胎细胞上主要的可碘化细胞表面蛋白。
