通过蛋白质工程和固定化策略提高有机磷水解酶的效力。
Enhancing organophosphate hydrolase efficacy via protein engineering and immobilization strategies.
机构信息
Department of Chemical and Biomolecular Engineering, New York University, Tandon School of Engineering, Brooklyn, New York.
Department of Radiology, New York University Langone Health, New York, New York.
出版信息
Ann N Y Acad Sci. 2020 Nov;1480(1):54-72. doi: 10.1111/nyas.14451. Epub 2020 Aug 19.
Organophosphorus compounds (OPs), developed as pesticides and chemical warfare agents, are extremely toxic chemicals that pose a public health risk. Of the different detoxification strategies, organophosphate-hydrolyzing enzymes have attracted much attention, providing a potential route for detoxifying those exposed to OPs. Phosphotriesterase (PTE), also known as organophosphate hydrolase (OPH), is one such enzyme that has been extensively studied as a catalytic bioscavenger. In this review, we will discuss the protein engineering of PTE aimed toward improving the activity and stability of the enzyme. In order to make enzyme utilization in OP detoxification more favorable, enzyme immobilization provides an effective means to increase enzyme activity and stability. Here, we present several such strategies that enhance the storage and operational stability of PTE/OPH.
有机磷化合物（OPs）作为杀虫剂和化学战剂开发，是具有极高毒性的化学物质，对公共健康构成威胁。在不同的解毒策略中，有机磷水解酶引起了广泛关注，为那些接触过 OPs 的人提供了一种解毒的潜在途径。磷酸三酯酶（PTE），也称为有机磷水解酶（OPH），是一种已被广泛研究的催化生物清除剂。在这篇综述中，我们将讨论针对提高酶的活性和稳定性的 PTE 蛋白质工程。为了使酶在 OP 解毒中的利用更加有利，酶固定化提供了一种增加酶活性和稳定性的有效手段。在这里，我们提出了几种增强 PTE/OPH 储存和操作稳定性的策略。
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