Kakegawa T, Takamiya K, Ogawa T, Hayashi Y, Hirose S, Niitsu M, Samejima K, Igarashi K
Faculty of Pharmaceutical Sciences, Chiba University, Japan.
Arch Biochem Biophys. 1988 Mar;261(2):250-6. doi: 10.1016/0003-9861(88)90339-6.
Various polyamine analogs were examined for their ability to stimulate and to function as sparing agents for the Mg2+ requirement in polypeptide synthesis at various temperatures in Escherichia coli (37 and 47 degrees C) and the extremely thermophilic Thermus thermophilus (60 and 70 degrees C) cell-free systems. The optimal concentration of each polyamine analog increased as the incubation temperature was elevated. At a fixed temperature, the optimal concentration of polyamine analogs was in the order diamines greater than triamines greater than tetraamines greater than pentaamines. All diamines tested stimulated polypeptide synthesis almost equally but lowered the optimal Mg2+ concentration in the order diaminopropane greater than putrescine greater than cadaverine. The degree of diamine stimulation was maximal at 37 degrees C. The effects of three triamines were very similar in the E. coli system but in the T. thermophilus system spermidine was most effective in stimulation of polypeptide synthesis. From the results of experiments using tetraamines and pentaamines, it was deduced that the presence of both aminobutyl and aminopropyl groups in polyamine analogs is important for stimulation of polypeptide synthesis. In the E. coli system, triamines were the most effective polyamines for stimulation of polyphenylalanine synthesis at both 37 and 47 degrees C, while, in the T. thermophilus system, thermospermine, a tetraamine, was most effective at 60 degrees C and 3,4,4,3-pentaamine was most effective at 70 degrees C.
研究了多种多胺类似物在大肠杆菌（37℃和47℃）以及嗜热栖热菌（60℃和70℃）无细胞体系中不同温度下刺激多肽合成以及作为Mg2+需求节省剂发挥作用的能力。随着孵育温度升高，每种多胺类似物的最佳浓度都有所增加。在固定温度下，多胺类似物的最佳浓度顺序为二胺大于三胺大于四胺大于五胺。所有测试的二胺对多肽合成的刺激作用几乎相同，但降低最佳Mg2+浓度的顺序为二氨基丙烷大于腐胺大于尸胺。二胺刺激程度在37℃时最大。三种三胺在大肠杆菌体系中的作用非常相似，但在嗜热栖热菌体系中，亚精胺对多肽合成的刺激最有效。从使用四胺和五胺的实验结果推断，多胺类似物中氨基丁基和氨基丙基的同时存在对于刺激多肽合成很重要。在大肠杆菌体系中，三胺是在37℃和47℃时刺激聚苯丙氨酸合成最有效的多胺，而在嗜热栖热菌体系中，四胺热精胺在60℃时最有效，3,4,4,3 - 五胺在70℃时最有效。
