多胺对人-人杂交瘤 HB4C5 细胞增殖和 IgM 产生的影响。
Effects of polyamines on proliferation and IgM productivity of human-human hybridoma, HB4C5 cells.
机构信息
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan,
出版信息
Cytotechnology. 2008 Jun;57(2):115-22. doi: 10.1007/s10616-007-9115-y. Epub 2007 Dec 21.
Immunoglobulin production stimulating activity of polyamines was investigated. Spermidine, thermine and triethylenetetraamine (TETA) stimulated IgM production of human-human hybridoma, HB4C5 cells under serum-free condition. IgM production of HB4C5 cells was accelerated 5.9-, 5.3-, and 3.7-fold by spermidine at 4.5 mM, thermine at 2 mM and TETA at 2.5 mM, respectively. However, putrescine did not enhance IgM production. Spermidine enhanced IgM productivity of the hybridoma cells in spite of its growth suppression activity. TETA also inhibited cell proliferation and the effect on the acceleration of IgM productivity disappeared during 5 days because of its cytotoxicity. On the other hand, thermine facilitated IgM productivity of the hybridoma cells without growth suppression. The laser confocal microscopic analysis revealed that IgM content inside HB4C5 cells was increased by thermine. This result suggests that thermine facilitates IgM synthesis in hybridoma cells.
研究了多胺的免疫球蛋白产生刺激活性。在无血清条件下，亚精胺、精胺和三乙烯四胺（TETA）刺激人-人杂交瘤 HB4C5 细胞的 IgM 产生。亚精胺在 4.5 mM 时、精胺在 2 mM 时和 TETA 在 2.5 mM 时分别将 HB4C5 细胞的 IgM 产生加速了 5.9、5.3 和 3.7 倍。然而，腐胺并没有增强 IgM 的产生。尽管亚精胺具有抑制细胞生长的活性，但它仍能增强杂交瘤细胞的 IgM 生产力。TETA 也抑制细胞增殖，并且由于其细胞毒性，在 5 天内加速 IgM 生产力的效果消失。另一方面，精胺促进杂交瘤细胞的 IgM 生产力而不抑制细胞生长。激光共聚焦显微镜分析显示，精胺增加了 HB4C5 细胞内的 IgM 含量。这一结果表明，精胺促进了杂交瘤细胞中 IgM 的合成。
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