Department of Gynecology, Federal University of São Paulo, São Paulo, SP, Brazil.
Department of Gynecology and Obstetrics, Rey Juan Carlos University, Madrid, Spain.
Gynecol Endocrinol. 2021 Jun;37(6):523-527. doi: 10.1080/09513590.2020.1810656. Epub 2020 Aug 21.
Mitochondria are known to play a key role in the regulation of reproductive capacity. Senescence is known to impair mitochondrial function and, ultimately, cellular energetic metabolism. Therefore, as women age, a deficient energy supply is likely to affect oocyte quality. The analysis of granulosa cells is considered a valuable noninvasive strategy to assess factors implicated in oocyte competence. Thus, we conducted an observational prospective cohort to evaluate the impact of aging on energy production by luteinized granulosa cells (LGCs). The control group comprised 13 young oocyte donors, whereas the comparison group included 13 infertile women over 38 years of age undergoing fertilization. Women with diseases that could potentially impact mitochondrial function were excluded. No differences were detected in the ATP levels in LGCs from young donors and infertile patients of advanced reproductive age (1.9 ± 0.99 picomoles in the control group vs. 2.1 ± 0.59 picomoles; -value = .139). Likewise, the ATP levels in our series did not correlate with either oocyte number or maturity. Despite the similar ATP levels in LGCs, an age effect on the bioenergetic status cannot be excluded. Energy metabolism is very complex, and ATP does not seem to be the most important and reliable parameter.
线粒体在调节生殖能力方面起着关键作用。衰老会损害线粒体功能，并最终影响细胞的能量代谢。因此，随着女性年龄的增长，能量供应不足可能会影响卵母细胞的质量。分析颗粒细胞被认为是评估与卵母细胞功能相关因素的一种有价值的非侵入性策略。因此，我们进行了一项观察性前瞻性队列研究，以评估衰老对黄体化颗粒细胞（LGC）能量产生的影响。对照组包括 13 名年轻卵母细胞供体，而比较组包括 13 名年龄超过 38 岁的不孕妇女进行受精。排除了可能影响线粒体功能的疾病的妇女。年轻供体和高龄不孕患者的 LGC 中 ATP 水平无差异（对照组为 1.9±0.99 皮摩尔，比较组为 2.1±0.59 皮摩尔；-值=.139）。同样，我们系列中的 ATP 水平与卵母细胞数量或成熟度也没有相关性。尽管 LGC 中的 ATP 水平相似，但不能排除年龄对生物能量状态的影响。能量代谢非常复杂，ATP 似乎不是最重要和最可靠的参数。
